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Dp soft image system

Manufactured by Nikon
Sourced in Japan

The DP-Soft image system is a software application developed by Nikon for image capture and analysis. It provides a user-friendly interface for controlling various Nikon digital cameras and microscopes, allowing users to capture, process, and analyze images. The core function of the DP-Soft image system is to facilitate the acquisition, storage, and manipulation of digital images for research, industrial, or educational purposes.

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3 protocols using dp soft image system

1

Immunohistochemical Analysis of Stomach Muscles

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A quantitative and statistical analysis of immunohistochemical reaction using the Olympus DP-Soft image system (version 4.1 for Windows) was carried out on a Microphot-SA (Nikon, Japan) microscope equipped with a Camedia-5050 Z digital camera (Olympus, Japan). The analysis was performed on sections from the SM strips from the stomach of Wistar rats (n = 6 for each group). Five sections of the SM strips were measured, and the percentage of cells expressing 5-HT3 in the circular and longitudinal layer of SM cells, as well as in the myenteric plexus of the stomach, was determined. Each antibody was analyzed for five fields, in each of them the average number of cells with positive unit area response at ×200 magnification.
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2

Quantitative Analysis of 5-HT3 Expression

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A quantitative and statistical analysis of immunohistochemical reaction using the Olympus DP-Soft image system (version 4.1 for Windows) was carried out on a Microphot-SA (Nikon, Tokyo, Japan) microscope equipped with a Camedia-5050Z digital camera (Olympus, Tokyo, Japan). The analysis was performed on sections from the SM strips from the stomach of Wistar rats (n = 6 for each group). Five sections of the SM strips were measured, and the percentage of cells expressing 5-HT3 in the circular and longitudinal layer of SM cells, as well as in the myenteric plexus of the stomach, was determined. Each antibody was analyzed for five fields, in each of them the average number of cells with positive unit area response at ×400 magnification.
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3

Immunohistochemical Analysis of Rat Testes

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Quantitative analysis of the immunohistochemical reaction was conducted using the Olympus DP-Soft image system (version 4.1 for Windows) and Microscope-SA (Nikon, Japan) system equipped with Camedia-5050Z digital camera (Olympus, Japan). The analysis was performed on sections from testis of Wistar rats (n=25 for each group of animals, controls, and the group fed the high lipid diet). The parameters were measured on 5 sections of the animal; we determined the percentage of cells expressing CRP, SAA, IL-4, and ACE in the seminiferous tubules and the testicular interstitial cells. For each antibody was analyzed for 5 fields of the testis of each animal in each of them by means of a graduated grating (6×5 fields, each field having a size of 100 μm 2 ) was determined average number of cells with positive unit area response at ×400 magnification.
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