The particle core size was determined from TEM micrographs acquired on a JEOL-1011 transmission electron microscope (100 kV). After being extracted from endolysosomes the particles were dispersed in water, and a drop of the suspension was placed onto a copper grid, covered by a carbon film and allowed to dry at RT. Microscopy images were taken by transmission electron TEM (a total of 30 images per type of MNPs and iron oxide core) of both the magnetic core and the MNPs once coated and the size of the magnetic core, their shape and distribution were analyzed using Image J software counting between 100 and 200 particles per image.
1011 transmission
Manufactured by JEOL
Sourced in Japan
The JEOL 1011 is a transmission electron microscope (TEM) designed for imaging and analysis of a wide range of samples. It provides high-resolution imaging capabilities with an accelerating voltage of up to 100 kV. The instrument is equipped with advanced optics and a digital imaging system for efficient data acquisition and analysis.
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Lab products found in correlation
7 protocols using 1011 transmission
1
Magnetic Nanoparticle Characterization by TEM
2
Preparing Liver Samples for TEM
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Selected sections of formalin-fixed liver were trimmed for electron microscopy, post fixed in a mixed aldehyde fixative followed by osmium tetroxide, contrasted in ethanolic uranyl acetate, dehydrated in an ascending series of ethanol, infiltrated in a mixture of propylene oxide and resin, and embedded into EMBed 812 resin. Thin sections (~80 nm) were mounted on copper EM support grids and counter stained with uranyl and lead salts. Samples were examined on a JEOL 1011 transmission electron microscope at 80kV. All supplies for electron microscopy were from Electron Microscopy Sciences (Hatfield, PA) unless otherwise noted.
3
Visualizing Extracellular Vesicle Morphology by TEM
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The morphology of EVs was examined by TEM. Samples were prepared according to Théry et al. 2006 with minor modifications [25 (link)]. Briefly, 2 µL of a sample was placed onto the surface of a formvar-coated grid, and was incubated for 10 min at RT. The residual solution was removed and fixed with 2% glutaraldehyde for 10 min. The grids were washed three times for 5 min, and we used uranyl oxalate for 10 min for contrast enhancement. The samples were further contrasted and embedded in a mixture of 4% uranyl acetate and 2% methyl cellulose and were examined by JEOL 1011 transmission electron microscope (Japan). HEK293T-palmGFP-derived mEVs were also visualized using a different approach. Transmission electron microscopy of ultrathin sections of EV pellets was carried out (Supplementary Fig. 5). In this case, the mEVs of the 12.5 k pellet were processed as described previously [26 (link)].
4
Transmission Electron Microscopy of Mitochondria
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The preparation of cells for transmission electron microscopy was performed as described previously [45 (link)]. Briefly, NCI-H460 and A549 cells were plated onto tissue culture-treated plastic. Seventy-two hours later, cells were rinsed quickly in warm Sorensen’s Buffer and fixed in pre-warmed (37 °C) 2.5% glutaraldehyde/ 2% paraformaldehyde solution (Proscitech, Kirwan, Australia) in Sorensen’s Buffer for 1 h at room temperature. Cells were further processed as described previously [46 (link)]. 60 nm ultrathin sections were cut on an Ultracut 6 (Leica Microsystems, Singapore) ultramicrotome. Grids were imaged at 80 kV on a JEOL 1011 transmission electron microscope fitted with a Morada 4 K × 4 K Soft Imaging Camera at two-fold binning (Olympus, Hong Kong). Mitochondrial volume density as a percentage of cytoplasmic volume was determined by stereology using point counting with a double lattice grid on systematically captured random images. Percentage mitochondrial area was averaged for 8–23 cells per biological replicate for n = 3 (A549) and n = 2 (NCI-H460) biological replicates (separate cell cultures).
5
Chlamydia psittaci Ultrastructural Analysis
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C. psittaci 02DC15-infected cells were fixed with 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 1 h. Postfixation was performed with 1% OsO4 in 0.1 M cacodylate buffer for 2 h. Samples were dehydrated with a graded ethanol series and embedded in araldite (Fluka, Buchs, Switzerland). Ultrathin sections were stained with uranyl acetate and lead citrate and were examined with a JEOL 1011 transmission electron microscope (TEM) (JEOL, Tokyo, Japan).
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6
Magnetic Nanoparticle Characterization by TEM
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The particle core size was determined from TEM micrographs acquired on a JEOL-1011 transmission electron microscope (100 kV). After being extracted from endolysosomes the particles were dispersed in water, and a drop of the suspension was placed onto a copper grid, covered by a carbon film and allowed to dry at RT. Microscopy images were taken by transmission electron TEM (a total of 30 images per type of MNPs and iron oxide core) of both the magnetic core and the MNPs once coated and the size of the magnetic core, their shape and distribution were analyzed using Image J software counting between 100 and 200 particles per image.
7
Ultrastructural Analysis of Gonadal Tissue
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Small pieces of gonadal area tissue were sampled and immersed for 16 h at 4 °C in a fixative solution (2.5% glutaraldehyde, 0.31 M cacodylate, 0.25 M saccharose, pH 7.4). The samples were rinsed in 0.38 M cacodylate buffer with 0.28 M saccharose at 4 °C and post-fixed for 2 h with 1% osmium tetroxide in 0.2 M cacodylate buffer containing 0.365 M saccharose. Tissues were dehydrated in ascending acetone concentrations and embedded in Epon. Ultrathin sections were mounted on coated copper grids and contrasted with uranyl acetate followed by lead citrate. Ultrathin sections were examined with a JEOL1011 transmission electron microscope. Image processing was done using Fiji software.
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