Stemfit ak01

Manufactured by Ajinomoto

Sourced in Japan

StemFit AK01 is a serum-free, xeno-free, and feeder-free culture medium for the maintenance and expansion of human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells.

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Lab products found in correlation

Stemfit, by Ajinomoto (1 mentions) Imatrix, by Nippi (1 mentions) Basic fibroblast growth factor (bfgf), by Fujifilm (1 mentions) Laminin 511 e8, by Nippi (1 mentions)

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StemFit (27 citations)

2 protocols using stemfit ak01

Generation of Human iPSCs from PBMCs

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Human iPSCs were generated from peripheral blood mononuclear cells (PBMCs) using episomal vectors (Sox2, Klf4, Oct3/4, L-Myc, Lin28, and p53-shRNA) as reported previously^{29 (link)} and cultured by a feeder-free culture system with StemFit (Ajinomoto, Tokyo, Japan). Karyotype analysis of iPSCs was conducted by LSI Medience (Tokyo, Japan). Established iPSCs were cultured under feeder-free conditions on iMatrix (Nippi, Tokyo, Japan)-coated plates with StemFit AK01 (Ajinomoto).

Sawamura M., Imamura K., Hikawa R., Enami T., Nagahashi A., Yamakado H., Ichijo H., Fujisawa T., Yamashita H., Minamiyama S., Kaido M., Wada H., Urushitani M., Inoue H., Egawa N, & Takahashi R. (2022). Cellular analysis of SOD1 protein-aggregation propensity and toxicity: a case of ALS with slow progression harboring homozygous SOD1-D92G mutation. Scientific Reports, 12, 12636.

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Generating iPSCs from ALS Patients

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iPSCs were generated from skin fibroblasts or peripheral blood mononuclear cells of sporadic ALS patients using episomal vectors (Sox2, Klf4, Oct3/4, L-Myc, Lin28, and p53-short hairpin RNA) as reported previously.²¹ (link)^,²² (link) Established iPSCs were cultured on an SNL feeder layer with human iPSC medium (primate embryonic stem cell medium; ReproCELL, Yokohama, Japan) supplemented with 4 ng/mL basic fibroblast growth factor (Wako Chemicals, Osaka, Japan) and penicillin/streptomycin or cultured under feeder-free conditions on laminin-511-E8 (Nippi, Tokyo, Japan)-coated plates with StemFit AK01 (Ajinomoto, Tokyo, Japan). The information on iPSC clones is listed in Table S3. Karyotyping was performed by Nihon Gene Research Laboratories (Sendai, Japan).

Hidaka T., Imamura K., Hioki T., Takagi T., Giga Y., Giga M.H., Nishimura Y., Kawahara Y., Hayashi S., Niki T., Fushimi M, & Inoue H. (2020). Prediction of Compound Bioactivities Using Heat-Diffusion Equation. Patterns, 1(9), 100140.

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