The levels of the CD44+, CD24 -, and CD133+cells in each group were evaluated using flow cytometry with a fluorescein isothiocyanate (FITC)-conjugated CD44 antibody (cat. no. 347943; BD Biosciences), phycoerythrin (PE)-conjugated CD24 antibody (cat. no. 555428; BD Biosciences), and a PE-conjugated CD133 antibody (cat. no. 12-1331-82; eBioscience; Thermo Fisher Scientific, Inc.). Mouse immunoglobulin (Ig)G2a-FITC (cat. no. 555573; BD Biosciences), mouse IgG2a-PE (cat. no. 554648; BD Biosciences), and mouse IgG1K-PE (cat. no. 12-4714-42; eBiosciences Thermo Fisher Scientific, Inc.) were used as the isotype controls (all of antibodies diluted at 1:100 ratio). Cells were incubated with antibodies at 4˚C for 45 minutes and analyzed using FACSCaliburTM (Becton Dickinson). The raw data were analyzed using Flowing Software version 2.5.0 (Perttu Terho).
Pe conjugated cd133 antibody
Manufactured by BD
The PE-conjugated CD133 antibody is a laboratory reagent used for the detection and analysis of CD133-positive cells in various research applications. It is designed to bind specifically to the CD133 cell surface antigen, which is commonly used as a marker for various stem and progenitor cell populations. The PE (Phycoerythrin) fluorescent dye is conjugated to the antibody, allowing for the visualization and quantification of CD133-expressing cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Facscalibur, by BD (1 mentions)
Mouse igg2a pe, by BD (1 mentions)
Mouse igg1k pe, by BD (1 mentions)
N2 supplement, by Thermo Fisher Scientific (1 mentions)
Peg 8000, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
2 protocols using pe conjugated cd133 antibody
1
Quantifying Cancer Stem Cell Markers
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The levels of the CD44+, CD24 -, and CD133+cells in each group were evaluated using flow cytometry with a fluorescein isothiocyanate (FITC)-conjugated CD44 antibody (cat. no. 347943; BD Biosciences), phycoerythrin (PE)-conjugated CD24 antibody (cat. no. 555428; BD Biosciences), and a PE-conjugated CD133 antibody (cat. no. 12-1331-82; eBioscience; Thermo Fisher Scientific, Inc.). Mouse immunoglobulin (Ig)G2a-FITC (cat. no. 555573; BD Biosciences), mouse IgG2a-PE (cat. no. 554648; BD Biosciences), and mouse IgG1K-PE (cat. no. 12-4714-42; eBiosciences Thermo Fisher Scientific, Inc.) were used as the isotype controls (all of antibodies diluted at 1:100 ratio). Cells were incubated with antibodies at 4˚C for 45 minutes and analyzed using FACSCaliburTM (Becton Dickinson). The raw data were analyzed using Flowing Software version 2.5.0 (Perttu Terho).
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The levels of the CD44+, CD24 -, and CD133+cells in each group were evaluated using flow cytometry with a fluorescein isothiocyanate (FITC)-conjugated CD44 antibody (cat. no. 347943; BD Biosciences), phycoerythrin (PE)-conjugated CD24 antibody (cat. no. 555428; BD Biosciences), and a PE-conjugated CD133 antibody (cat. no. 12-1331-82; eBioscience; Thermo Fisher Scientific, Inc.). Mouse immunoglobulin (Ig)G2a-FITC (cat. no. 555573; BD Biosciences), mouse IgG2a-PE (cat. no. 554648; BD Biosciences), and mouse IgG1K-PE (cat. no. 12-4714-42; eBiosciences Thermo Fisher Scientific, Inc.) were used as the isotype controls (all of antibodies diluted at 1:100 ratio). Cells were incubated with antibodies at 4˚C for 45 minutes and analyzed using FACSCaliburTM (Becton Dickinson). The raw data were analyzed using Flowing Software version 2.5.0 (Perttu Terho).
2
Antibody Profiling for Cellular Characterization
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Commercial antibodies were mouse anti-β-actin, mouse anti-Flag, rabbit anti-C8orf4 antibodies (Sigma-Aldrich); mouse anti-green fluorescent protein (anti-GFP), PE-conjugated CD13 antibodies (Sungene Biotech, Tianjin); rabbit anti-NOTCH2 antibody (Cell Signaling Technology); PE-conjugated CD133 antibody (BD), Rabbit anti-CD133, anti-CD13 antibodies (Sangon Biotech, Shanghai); Calreticulin, Alexa488-conjugated goat anti-rat immunoglobulin-G (IgG; Biolegend); Alexa488-conjugated donkey anti-mouse IgG, Alexa594-conjugated donkey anti-mouse IgG, Alexa594-conjugated donkey anti-rabbit IgG (Molecular Probes) and horseradish peroxidase (HRP)-conjugated secondary antibody (Santa Cruz). Dilutions of anti-β-actin, anti-Flag, anti-GFP and HRP-conjugated secondary antibodies (for western blot) were 1:5,000, dilutions of anti-CD133, anti-CD13 and fluorescence-conjugated or HRP-conjugated secondary antibodies (for IHC staining) were 1:500, and the others were 1:1,000. Other major reagents were bFGF (Millipore), EGF (Sigma-Aldrich), N2 supplement (Life Technologies), B27 (Life Technologies), PEG8000 (Sigma-Aldrich), DAPI (4′,6-diamidino-2-phenylindole; Sigma-Aldrich) and PI (Sigma-Aldrich).
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