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Ficoll centrifugation

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Ficoll centrifugation is a technique used for the separation and purification of cells and other biological materials. It involves the use of a density gradient medium, Ficoll, which allows for the separation of different cell types or components based on their density during centrifugation. The core function of Ficoll centrifugation is to provide a gentle and effective way to isolate and enrich specific cell populations or subcellular fractions from a heterogeneous sample.

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Lab products found in correlation

Macs cd19 b or cd8 t cell isolation kit, by Miltenyi Biotec (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Collagenase 4, by Merck Group (1 mentions) Dnase 1, by Roche (1 mentions) Cd8 fitc, by Thermo Fisher Scientific (1 mentions) Cd4 apc cy7, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Ficoll-gradient centrifugation Ficoll density gradient centrifugation Ficoll

4 protocols using ficoll centrifugation

1

Isolation and Sorting of B and T Cells

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PBMCs were isolated from peripheral blood samples from healthy donors by Ficoll centrifugation (Axis-Shield). CD19+ B or CD8+ T cells (collected populations were more than 95% CD19+ or CD8+) were sorted from among the PBMCs using a MACS CD19+ B or CD8+ T cell Isolation Kit (Miltenyi Biotech).
Ye L., Li Y., Tang H., Liu W., Chen Y., Dai T., Liang R., Shi M., Yi S., Chen G, & Yang Y. (2019). CD8+CXCR5+T cells infiltrating hepatocellular carcinomas are activated and predictive of a better prognosis. Aging (Albany NY), 11(20), 8879-8891.
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2

Isolation of PBMCs and Tissue-Infiltrating Leukocytes

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Peripheral blood mononuclear cells (PBMCs) were isolated using a standard Ficoll procedure. Tissue-infiltrating leukocytes were dissociated from tissue specimens and collected as described previously [28 (link)]. Specimens were cut into small pieces and dissociated using RPMI 1640 medium (Gibco) supplemented with 0.05% collagenase IV (Sigma-Aldrich), 0.002% DNase I (Roche), and 20% FBS (Gibco) at 37 °C for 1 h. Dissociated cells were filtered through a 150-mm mesh and separated by Ficoll centrifugation (Axis-Shield), and the mononuclear cells were washed with HBSS and resuspended in RPMI 1640 medium supplemented with 10% FBS.
Ye L., Zhang Q., Cheng Y., Chen X., Wang G., Shi M., Zhang T., Cao Y., Pan H., Zhang L., Wang G., Deng Y., Yang Y, & Chen G. (2018). Tumor-derived exosomal HMGB1 fosters hepatocellular carcinoma immune evasion by promoting TIM-1+ regulatory B cell expansion. Journal for Immunotherapy of Cancer, 6, 145.
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3

Isolation and Coculture of Immune Cells

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Ficoll centrifugation (Axis-Shield) was used to isolate peripheral blood mononuclear from healthy donor blood samples. After 72 h of exposure to NorCA, LM3 cells or Exos were cocultured with CD4+ and CD8+ T cells that had been stimulated with anti-CD3/CD28 mAb beads. These results were analyzed by FlowJo 10.0 software. The fluorochrome-linked antibodies were applied as follow: anti-human Annexin V-FITC, PI-PE, CD4-APC-Cy7, CD8-FITC, PD-1-BV510, TIM3-PE, and CTLA4-PECY-Cy5.5 (eBioscience). Detailed staining protocols were followed previously described (21 (link)).
Gong Y., Li K., Qin Y., Zeng K., Liu J., Huang S., Chen Y., Yu H., Liu W., Ye L, & Yang Y. (2021). Norcholic Acid Promotes Tumor Progression and Immune Escape by Regulating Farnesoid X Receptor in Hepatocellular Carcinoma. Frontiers in Oncology, 11, 711448.
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4

Isolation of B and T Cells

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Peripheral blood mononuclear cells were isolated from healthy donor blood samples by Ficoll centrifugation (Axis-Shield). CD19+ B or CD8+ T cells (more than 95% CD19+ or CD8+) were purified from peripheral blood mononuclear cells using a MACS CD19+ B or CD8+ T cell Isolation Kit (Miltenyi Biotech).
Ye L., Zhang Q., Cheng Y., Chen X., Wang G., Shi M., Zhang T., Cao Y., Pan H., Zhang L., Wang G., Deng Y., Yang Y, & Chen G. (2018). Tumor-derived exosomal HMGB1 fosters hepatocellular carcinoma immune evasion by promoting TIM-1+ regulatory B cell expansion. Journal for Immunotherapy of Cancer, 6, 145.
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