Bupropion hydrochloride and naltrexone-hydrochloride (Sigma Aldrich, USA) or HS014 (Tocris, USA) were dissolved in 0.9% saline and administered within a range of doses known to affect alcohol drinking or food consumption in rodents [Greenway et al., 2009 (link); Zhou et al., 2017a (link); Levy et al., 2018 (link)]. The BPP+NTX combination was studied at a ratio of 10 BPP/1 NTX in most experiments to model the ratio employed in Contrave® [Greenway et al., 2009 (link)]. The alcohol solution from 190 proof absolute ethyl alcohol (Pharmco-AAPER, Brookfield, CT), sucrose or saccharin [Sigma-Aldrich Inc., St. Louis, MO, USA) was diluted in tap water.
Hs014
Manufactured by Bio-Techne
Sourced in United Kingdom
The HS014 is a high-sensitivity ELISA kit developed by Bio-Techne. The kit is designed to detect and quantify specific analytes in biological samples. It provides a reliable and accurate measurement tool for research applications.
Automatically generated - may contain errors
Lab products found in correlation
Naltrexone hydrochloride, by Merck Group (1 mentions)
Bupropion hydrochloride, by Merck Group (1 mentions)
6 cyano 7 nitroquinoxaline 2 3 dione cnqx, by Bio-Techne (1 mentions)
α msh, by Bio-Techne (1 mentions)
Bicuculline, by Bio-Techne (1 mentions)
Agrp 83 132 amide, by Phoenix Pharmaceuticals (1 mentions)
α flupenthixol dihydrochloride, by Merck Group (1 mentions)
Biie0246, by Bio-Techne (1 mentions)
Bibo3304, by Bio-Techne (1 mentions)
α msh, by Abcam (1 mentions)
Psn632408, by Cayman Chemical (1 mentions)
Bombesin, by Merck Group (1 mentions)
Human α msh, by Merck Group (1 mentions)
D glucose, by Merck Group (1 mentions)
4 protocols using hs014
1
Rodent Alcohol and Food Consumption Study
2
Intra-VTA drug infusion protocol for behavioral testing
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αMSH (Tocris, UK), HS014 (Tocris, UK), D-trp8-γMSH (γMSH; Tocris, UK,), AGRP (83–132)-amide (Phoenix Pharmaceuticals, USA), and α-flupenthixol dihydrochloride (Sigma Aldrich, USA) were dissolved in sterile saline. 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, Tocris, UK), DL-2-Aminophosphonopentanoic acid (APV, Tocris, UK), and bicuculline (Tocris, UK) were prepared as a stock solution.
For i.c.v. infusions, rats were briefly restrained and 2 μl of drug solution was injected over 10 s into the ventricular cavity. Intra-VTA infusions were performed through an injector (9 mm, 33GA, Plastics One, USA) inserted into the guide cannula. Bilateral infusions (300 nl over 30 s) were made using a syringe pump with the injectors left in place for another 30 s to allow for diffusion. α-Flupenthixol dihydrochloride was injected intraperitoneally (i.p.) 30 min before intra-VTA infusions. All animals received all drug doses/combinations, according to a Latin-square design. Behavioral testing commenced 5 min after drug infusions and a minimum 1 day drug-free period was maintained between infusions during which the animals were trained but not tested.
For i.c.v. infusions, rats were briefly restrained and 2 μl of drug solution was injected over 10 s into the ventricular cavity. Intra-VTA infusions were performed through an injector (9 mm, 33GA, Plastics One, USA) inserted into the guide cannula. Bilateral infusions (300 nl over 30 s) were made using a syringe pump with the injectors left in place for another 30 s to allow for diffusion. α-Flupenthixol dihydrochloride was injected intraperitoneally (i.p.) 30 min before intra-VTA infusions. All animals received all drug doses/combinations, according to a Latin-square design. Behavioral testing commenced 5 min after drug infusions and a minimum 1 day drug-free period was maintained between infusions during which the animals were trained but not tested.
3
Detailed Compound Solubilization and Storage
4
Melanocortin Receptor Agonist Assay
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Nle(4),D-Phe(7)]α-melanocyte-stimulating hormone (NDP-α-MSH) was from Phoenix Pharmaceuticals, Inc. (Cat. no. 043-06, Burlingame, CA, USA). HS014 was from Tocris (Cat. no. 1831, Abingdon, UK). Human α-MSH, D-glucose and bombesin were from Sigma Aldrich (Cat. no. M4135, G8270 and B4272, Brøndby, Denmark).
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