Blood samples after a 12 h overnight fast were collected at baseline and 4 and 12 weeks after the randomization for general blood testing, biochemical testing, and lipid testing to evaluate metabolic risk factors and monitor on potential adverse effects of LLE. The serum creatinine and blood pyruvate levels were measured by Jaffe's kinetic alkaline picrate method and the enzymatic method, respectively. Lactate concentrations and serum electrolytes levels were measured using an ion-selective electrode, while serum creatinine kinase (CK) concentration was measured using the kinetic ultraviolet method. Liver enzyme and total cholesterol levels were measured with Toshiba TBA200FR (Toshiba Co. Ltd., Tokyo, Japan) using an enzymatic colorimetric method, and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol were measured with Toshiba TBA200FR directly. Triglycerides were measured using lipase, glycerol kinase (GK), glycerol-3-phosphate oxidase (GPO), and peroxidase (POD) with a glycerol blank. Fasting blood sugar was measured using a glucose oxidase test method (LX-20, Beckman Coulter, Fullerton, CA, USA), and the serum insulin concentration was measured using Coat-A-Count® Insulin with solid-phase 125I radioimmunoassay.
Tba 200fr
Manufactured by Toshiba
Sourced in Japan
The TBA-200FR is a laboratory equipment product manufactured by Toshiba. It serves as a tool for researchers and scientists, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Additional information about the intended use or features of this product is not available.
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28 protocols using tba 200fr
1
Metabolic Risk Factors Assessment
2
Anthropometric and Biochemical Measurements
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Height, weight, and abdominal circumference were measured separately and body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared. Blood pressure (BP) was measured with automatic machine (BP-203 RVII; Colin Corp., Aichi, Japan) in sitting position after taking 10 min break. Systolic and diastolic pressure were measured once and recorded. The blood test was done after 8 hr of overnight fasting. Total cholesterol and uric acid were measured by using Toshiba TBA200FR (TOSHIBA Co., Tokyo, Japan) with enzymatic colorimetric method. Intra-assay and inter-assay coefficients of variation of uric acid were 1.0% and 1.3%. Low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol were directly measured by using Toshiba TBA200FR. Fasting glucose was measured by using glucose oxidase (LX-20; Beckman Coulter, Brea, CA, USA). Coat-A-Count® Insulin was used to measure insulin concentration by solid-phase 125I radioimmunoassay. Thyroid stimulating hormone (TSH) was measured by Coat-A-Count TSH immunoradiometric assay (IRMA), Siemens, and free thyroxine (FT4) was measured by using Coat-A-Count FT4, Siemens. Homeostasis model assessment (HOMA)-IR was calculated by using glucose and insulin level (HOMA-IR = [glucose × insulin] / 405).
3
Evaluating Safety of Bowel Prep Agents
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In order to evaluate the safety of each bowel preparation agents, especially NaP tablets, laboratory tests were conducted three times in all of the participants: 1) baseline (the day of enrollment and allocation); 2) visit 1 (the day of the colonoscopy and post-preparation); and 3) visit 2 (1 week follow-up after colonoscopy). Blood samples were taken from the antecubital vein of subjects and measured using an automatic analyzer (Toshiba TBA 200FR, Toshiba Medical Systems Co. Ltd., Tokyo, Japan). The quantity levels of protein and blood in urine were assessed using Uropaper (US-3100R, Eiken Chemical, Tokyo, Japan). Estimated glomerular filtration rate (eGFR) was calculated using the Modification of Diet in Renal Disease (MDRD) study equation as an indicator of renal function: eGFR (milliliter per minute per 1.73 m2)=186.3×(serum creatinine)-1.154×(age)-0.203 (×0.742 if the subject was female).
4
Serum Biomarker Quantification Protocol
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Blood samples (10 ml) were collected from the vena cava at sacrifice, and the serum was separated. All serum samples were frozen at −40°C until required for further experimentation. Serum levels of osteocalcin (ng/ml) were detected using an Osteocalcina Myria kit (Technogenetics Srl, Milan, Italy) and a Packard Cobra II γ-counter (GMI, Inc., Ramsey, MN, USA). Serum levels of bALP (U/L) were detected using the commercially available Enzyme-Immunoassay kit (Metra™ bALP kit; cat. no. 8012; Quidel Corporation, San Diego, CA, USA). The serum calcium (Ca) levels (mg/ml) were detected using the Orthocresolphthalein Complexone (OCPC) method and an automated blood analyzer (TBA 200FR; Toshiba, Tokyo, Japan). Briefly, the OCPC complexone (P5631; Sigma-Aldrich) method is based on the reaction of Ca2+ with o-cresolphthalein complexone in an alkaline solution, which forms an intense violet fluorescence which maximally absorbs at 577 nm. 8-hydroxyquionline is added to prevent interference by magnesium and iron. The serum phosphorus (P) levels were detected using the kinetic ultraviolet (UV) method using a blood biochemistry autoanalyzer (Dri-Chem NX500i; Fujifilm Medical System Co., Ltd., Tokyo, Japan).
5
Fasting Blood Analysis Protocol
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Blood was drawn at the antecubital vein from all subjects between 8:30 and 10:00 am following at least eight hours of fasting, and we analyzed Cys-C using turbidimetric immunoassay. The liver and renal function tests included alanine aminotransferase, aspartate aminotransferase, lipid profiles, blood urea nitrogen (BUN), serum creatinine, and total bilirubin as well as others, all of which we measured using the Toshiba TBA200FR (Toshiba Co., Tokyo, Japan).
6
Quantifying Serum Vitamin D Biomarkers
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Each serum sample was aliquoted into two tubes and stored at -80℃ until they could be analyzed for VDBP and total 25(OH)D. Concentrations of VDBP were measured by using Human Vitamin D BP Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol. The 25(OH)D2 and 25(OH)D3 concentrations were measured by using liquid chromatography with tandem mass spectrometry (LC-MS/MS). LC-MS/MS was regarded as a reference method and was performed at a College of American Pathologists-accredited laboratory. The D2 and D3 values were added up to determine total 25(OH)D. The coefficients of variation of D2 and D3 measured by LC-MS/MS were as follows; D2: 4.2% for a low level, 3.8% for a high level; D3: 4.0% for a low level, 3.5% for a high level. Serum albumin levels were measured by using an enzymatic colorimetric method with a TBA-200FR (Toshiba, Tokyo, Japan). The levels of bioavailable 25(OH)D were calculated from total measured 25(OH)D, VDBP, and serum albumin concentrations by using the following equations [4 (link)8 (link)]:
7
Comprehensive Blood Biomarker Profiling
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Fasting blood samples were obtained in the morning after a 12-h fast including any medication. The glycated hemoglobin (A1c) level was measured by affinity chromatography (Bio-Rad Laboratories, Hercules, CA, USA). A complete blood cell count analysis was performed using Sysmex XE-2100 (Sysmex, Mundelein, IL, USA). Fasting plasma concentrations of total cholesterol, triglycerides, high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol, and serum creatinine were measured on a Hitachi 747 chemistry analyzer (Hitachi, Tokyo, Japan). Estimated glomerular filtration rate (eGFR) was calculated using the Modification of Diet in Renal Disease equation [20 (link)]. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured with an autoanalyzer (TBA-200FR, Toshiba, Tokyo, Japan).
8
Mouse Plasma ALT Measurement
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Mouse plasma samples were collected, and the concentrations of ALT were determined using GPT-JS kits (Denka Seiken, Tokyo, Japan) and an automated biochemical analyzer (model TBA-200FR; Toshiba, Tokyo, Japan).
9
Comprehensive Metabolic and Thyroid Panel
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We measured the subjects’ height and weight while wearing light clothing and without shoes to the nearest 0.1 cm and 0.1 kg, respectively. Body mass index (BMI) was calculated by determining the ratio between weight and the square of the height and expressed in kg/m2. Right arm blood pressure was measured with the subject in a seated position after at least 10 minutes of resting. Serum levels of blood urea nitrogen, creatinine, and glucose were measured by automated standard laboratory methods (Hitachi 747, Hitachi, Tokyo, Japan). Serum total protein, albumin, total bilirubin, alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase were measured with an autoanalyzer (TBA-200FR, Toshiba, Tokyo, Japan). For TFT, concentrations of serum free thyroxine (T4; DiaSorin, Saluggia, Italy) and TSH (CIS Bio International, Gif-sur-Yvette, France) were measured using immunoradiometric assays. The reference ranges for free T4 and TSH were 0.89–1.79 ng/dL and 0.3–4.0 mIU/L, respectively. Hypothyroidism was defined based on the results of the TFT, as low free T4. TSH was not used for defining hypothyroidism because subjects in the control group used rhTSH, which increased serum TSH levels, regardless of the peripheral action of thyroid hormone.
10
Metabolic Risk Factors Measurement
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The measurement of MRFs included WC, systolic blood pressure (SBP) and/or diastolic blood pressure (DBP), fasting glucose level (FBG), triglycerides (TG), and HDL-cholesterol (HDL-C) [43 (link),44 (link)]. The measurement of WC (cm) was performed by trained health personnel in accordance with the International Standards for Anthropometry and Kinesiology (ISAK) [45 ]. The BP measurement was taken using a validated digital sphygmomanometer (HEM-7310, Omron, Kyoto, Japan) while the participant was in a seated position after at least five minutes of rest. In addition, blood samples were collected after at least eight hours of overnight fasting, and the medical laboratory (certified ISO 15189) measured the biochemical parameters using a biochemical auto-analyzer (TBA-200FR, Toshiba, Tokyo, Japan). FBG was analyzed using an enzymatic UV test (hexokinase method). TG was measured using a series of coupled enzymatic reactions. HDL-C was measured by direct methods.
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