Mithramycin (Tocris Bioscience) was dissolved as a 1 mg/mL stock solution in PBS, and frozen in aliquots. PHA-767491 and other small molecule inhibitors (Selleck Chemicals) were dissolved in DMSO as stock solutions. Small molecule stocks were stored at −80 °C for storage or −20 °C for experimental use, minimizing freeze-thaw cycles. For in vivo experiments, a 30 mg/mL stock solution of PHA-767491 (Adooq Bioscience) was made by dissolving the powder in a solution of 1 mg/10mL D-α-Tocopherol polyethylene 1000 succinate (MilliporeSigma) for oral gavage.
Pha 767491
Manufactured by Adooq
PHA-767491 is a laboratory equipment product. It is a chemical compound used in research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
Mithramycin, by Bio-Techne (1 mentions)
Pha 767491, by Selleck Chemicals (1 mentions)
Small molecule inhibitors, by Selleck Chemicals (1 mentions)
D α tocopherol polyethylene 1000 succinate, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Iscript reverse transcription supermix for qrt pcr, by Bio-Rad (1 mentions)
Pgfp c shlenti, by OriGene (1 mentions)
Phosphatase inhibitor cocktail, by Merck Group (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Alamar blue, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 annexin 5 dead cell apoptosis kit, by Thermo Fisher Scientific (1 mentions)
Pageruler plus prestained protein, by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
β actin, by Merck Group (1 mentions)
Accutase solution, by Merck Group (1 mentions)
2 protocols using pha 767491
1
Preparation and Storage of Mithramycin and PHA-767491
2
CDC7 Inhibition Induces Apoptosis
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Following reagents and primary antibodies are used in this study: DMEM-F12 (Gibco, 10565-018), Fetal bovine serum (Gibco, 10082-147), Accutase solution (Sigma, A6964-100), Alamar Blue (Invitrogen, DAL1100), RIPA buffer (Sigma, R0278), Phosphatase inhibitor cocktail (Sigma, P0044), Protease inhibitor cocktail (P8340), Bradford (BIORAD, 500-0006), BSA used in Bradford assay (BioLabs, B9001S), PageRuler plus prestained protein (Thermo scientific, 26619), iScript Reverse Transcription supermix for qRT-PCR (Bio-rad, 170-8841), shCDC7 lentivirus particles (pGFP-C-shLenti, Origene, TL320578V), PHA-767491 (AdooQ, A11164-100), Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit (Thermo Fisher Scientific,V13241). anti-CDC7 (Novus Biologicals, DCS-341, Mouse, used for WB and IHC), anti-CDC7 (Abcam, ab108332, Rabbit, used for FACs), anti-RAD54L (Novus Biologicals, NBP2-33916, Rabbit, used for WB), anti-RAD54L (Novus Biologicals, NB100-148, Mouse, used for WB), anti- Cleaved Caspase 3 (Cell Signaling Technology, #9664, Rabbit, used for WB), β-Actin (Sigma, A5316, Mouse, used for WB).
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