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Application suite las advanced fluorescence

Manufactured by Leica
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Leica Application Suite (LAS) Advanced Fluorescence is a software package designed for the control and analysis of fluorescence microscopy data. It provides a comprehensive suite of tools for image acquisition, processing, and analysis.

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Lab products found in correlation

Sytox green nucleic acid staining, by Thermo Fisher Scientific (2 mentions) Dmi 400b inverted microscope, by Leica (2 mentions) Hoechst 33342 solution, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Dmi4000b, by Leica (1 mentions)

Close spelling variants of this product

Application Suite Advanced Fluorescence (LAS AF) software Leica Application Suite Advanced Fluorescence (LAS AF, Leica Application Suite (LAS) Advanced Fluorescence 2.7.3.9723 Leica Application Suite Advanced Fluorescence (LAS AP) software Leica Application Suite (LAS) Advanced Fluorescence Lite software Leica Application Suite for Advanced Fluorescence (LAS AF; version 2.35) software Leica Application Suite (LAS) Advanced Fluorescence Lite software (LAS AF Lite, Leica Application Suite Advanced Fluorescence Leica Application Suite Advanced Leica Application Suite (LAS).

4 protocols using application suite las advanced fluorescence

1

SYTOX Green Live Cell Staining

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SYTOX Green nucleic acid staining (Thermo Fisher Scientific) was performed following manufacturer’s suggestions, and adapted as follows: A final concentration of 0.1 μM SYTOX green was added directly to the media of live cells. In addition, Hoechst 33342 solution (Thermo Fisher Scientific) was added as a counterstain to label all nuclei at a final concentration of 1 μg/ml in culture media. Samples were incubated for at least 10 minutes in 37°C. Images were captured using a Leica DMI 400B inverted microscope with Leica Application Suite (LAS) Advanced Fluorescence. Three images were taken from random areas of each coverslip for at least three biological replicates per experiment. Quantification performed by counting number of SYTOX-positive cells over total Hoechst signal.
Victor M.B., Richner M., Olsen H.E., Lee S.W., Monteys A.M., Ma C., Huh C.J., Zhang B., Davidson B.L., Yang X.W, & Yoo A.S. (2018). Striatal neurons directly converted from Huntington’s disease patient fibroblasts recapitulate age-associated disease phenotypes. Nature neuroscience, 21(3), 341-352.
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2

SYTOX Green Live Cell Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
SYTOX Green nucleic acid staining (Thermo Fisher Scientific) was performed following manufacturer’s suggestions, and adapted as follows: A final concentration of 0.1 μM SYTOX green was added directly to the media of live cells. In addition, Hoechst 33342 solution (Thermo Fisher Scientific) was added as a counterstain to label all nuclei at a final concentration of 1 μg/ml in culture media. Samples were incubated for at least 10 minutes in 37°C. Images were captured using a Leica DMI 400B inverted microscope with Leica Application Suite (LAS) Advanced Fluorescence. Three images were taken from random areas of each coverslip for at least three biological replicates per experiment. Quantification performed by counting number of SYTOX-positive cells over total Hoechst signal.
Victor M.B., Richner M., Olsen H.E., Lee S.W., Monteys A.M., Ma C., Huh C.J., Zhang B., Davidson B.L., Yang X.W, & Yoo A.S. (2018). Striatal neurons directly converted from Huntington’s disease patient fibroblasts recapitulate age-associated disease phenotypes. Nature neuroscience, 21(3), 341-352.
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3

Live Cell Nucleic Acid Staining

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0.1 μM Sytox gene nucleic acid stain and 1 μl/mL of Hoechst 33342 Solution were added into the cell medium. Samples were incubated for at least 15 mins at 37 °C before imaging. Images were taken using Leica DMI 4000B inverted microscope with Leica Application Suite (LAS) Advanced Fluorescence.
Lee S.W., Oh Y.M., Victor M.B., Strunilin I., Chen S., Dahiya S., Dolle R.E., Pak S.C., Silverman G.A., Perlmutter D.H, & Yoo A.S. (2023). Longitudinal modeling of human neuronal aging identifies RCAN1-TFEB pathway contributing to neurodegeneration of Huntington’s disease. Research Square.
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4

Live-Cell Fluorescent Imaging

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0.1 μM SYTOX gene nucleic acid stain (Invitrogen, S7020) and 1 μl/mL of Hoechst 33342 (Thermo Scientific, 66249) were added into cell medium. Samples were incubated for at least 30 mins at 37°C prior to live-cell imaging. Images were taken using Leica DMI 4000B inverted microscope with Leica Application Suite (LAS) Advanced Fluorescence.
Oh Y.M., Lee S.W., Kim W.K., Chen S., Church V.A., Cates K., Li T., Zhang B., Dolle R.E., Dahiya S., Pak S.C., Silverman G.A., Perlmutter D.H, & Yoo A.S. (2022). Age-related Huntington’s disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy. Nature neuroscience, 25(11), 1420-1433.
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