M igg fc bp hrp sc 525409

Most of the material used in this study was purchased, as we reported earlier [38 (link),39 (link),40 (link),41 (link),42 (link),43 (link)]. Phosphate-buffered saline (PBS), nitrocellulose membranes, nicotine, BDNF, epinephrine, and propranolol hydrochloride were purchased from Sigma-Aldrich. The caspase 3 (cleaved) colorimetric In-Cell ELISA Kit (62218), Halt Protease and Phosphatase Inhibitor Cocktail, BCA protein assay kit, super signal west pico luminol (chemiluminescence) reagent, human IgG (hIgG) isotype control, α-tubulin monoclonal antibody (DM1A), goat anti-mouse IgG (H + L) superclonal secondary antibody, HRP conjugate (A28177), 3,3′,5,5′-tetramethylbenzidine (TMB), and lipofectamine 2000 transfection reagent were from ThermoFisher. Donkey anti-mouse IgG (HRP) (ab205724) was purchased from Abcam. MMP9 siRNA (sc-29400), MMP9 antibody (sc-393859), m-IgG Fc BP-HRP: sc-525409, and anti-E-cadherin antibody (DECMA-1, sc-59778) were from Santa Cruz Biotechnology. SignalSilence Control siRNA (unconjugated, 6568) was purchased from (Cell Signaling Technology, Danvers, MA, USA).

Histological analysis of target gene expression was performed via immunohistochemistry (IHC) in human kidney tissue (Cohort 3). Formalin-fixed paraffin-embedded (FFPE) sections of 5 µm were stained with mouse monoclonal antibodies against our target gene of interest. We purchased the primary FcER1 antibody (sc-390222) and secondary m-IgG Fc BP-HRP (sc-525409) from Santa Cruz Biotech (Dallas, TX) and used at a dilution of 1:100 with appropriate negative controls.