Amiloride hcl

Dulbecco’s Modified Eagle Medium (DMEM), heat-inactivated low-endotoxin fetal bovine serum (FBS), and gentamicin purchased from Cambrex (Walkersville MD, USA) for tissue culture. All other cancer cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). Most of these following compounds (thiostrepton, ampicillin, dexamethasone, 2-methoxyestradiol, (−) riboflavin, ascorbic acid, amiloride-HCL, (−) Corey lactone, and quinine sulphate) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA), and quercetin was purchased from Alfa Aesar (Johnson Matthey Co. Lancastor, UK). The 50% purified δ-tocotrienol fraction from annatto seeds was purchased from American River (Boston, MA, USA). RNeasy mini kit was purchased from QIAGEN Sciences (Germantown, MD, USA). DeltaGold (125 mg soft gels) from annatto seeds (composition 90% δ-tocotrienol + 10% γ-tocotrienol) was obtained as a gift by American River Nutrition, Inc. (Hadley, MA. USA). The hepatitis C antibody test kit was purchased from Sigma Chemical Co., St. Louis, USA. The second kit for diagnosing hepatitis C test is RNA (PCR) test. Pure total mRNA was obtained from the EDTA treated fresh whole blood using total RNA purification kit # 17200 (NORGEN Bioteck Corporation, Thorold, ON, Canada).

SPD (> 99% purity) was obtained from Sigma-Aldrich (St Louis, MO). SPD (2 μg/wound) was added to 100 μl of white petrolatum. The formulation was heated to 60 °C and quickly mixed to emulsify the components. Distilled water containing SPD (5 mM) was systemically administered daily for 4 days before skin wound creation. MDI-2268 obtained from AOBIOUS INC (Gloucester, MA) was dissolved in 0.1% DMSO in lactated ringer buffer. After a skin wound had been created, mice received MDI-2268 (3 mg/kg) or vehicle by intraperitoneal administration for 2 days. Amiloride HCl (a uPA inhibitor) was obtained from Sigma-Aldrich (St Louis, MO). After a skin wound had been created, mice were intraperitoneally administered Amiloride HCl (10 mg/kg) for every day.

L-lecithin, L-Valine, D-valine, thioacetic, 1-butanethiol, carbon disulfide (CS₂), ethyl 2-bromopropionate, thioacetic acid and acryloyl chloride were purchased from Aladdin company. Triethylamine (TEA), dimethyl formamide (DMF) and dichloromethane (DCM) were obtained from Sinopharm Chemical Regent Co., Ltd, and were vacuum-distilled prior to use. Gold (III) chloride hydrate (HAuCl₄), and trisodium citrate dihydrate (C₆H₅Na₃O₇·2H₂O) were purchased from Sinopharm group Co. Ld. Amiloride-HCl (Amilo), amantadine-HCl (Aman), sodium azide (NaN₃), genistein (Ge), Triton X-100, 4′,6-diamidino-2-phenylindole (DAPI), cytochalasin D (CytD) and 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma. Acryloyl-L(D)-valine monomers, and methyl 2-(butylthiocarbonothioylthio)propanoate (MCEBTTC) were synthesized and characterized in Supporting information. All other chemicals were of analytical grade and used without further treatment if not specially mentioned. The MilliQ water was used throughout the experiments. For AuNPs synthesis, all glasswares used were cleaned by freshly prepared aqua regia solution (HCl/HNO₃, 3:1).