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129s4 svjae

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The 129S4/SvJae is a strain of laboratory mice developed for research purposes. It is a specific inbred strain that has been used in various scientific studies. As a marketing specialist, I can provide a factual and unbiased description of the core function of this product without extrapolation or interpretation.

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Lab products found in correlation

129 svimj, by Jackson ImmunoResearch (2 mentions) 129s4 svjae pparαtm1gonz j female mice, by Jackson ImmunoResearch (2 mentions) C57bl 6j, by Jackson ImmunoResearch (2 mentions) Chromogenic assay, by Hyphen Biomed (1 mentions) Goat anti mouse mgl1 2 antibody, by R&D Systems (1 mentions)

4 protocols using 129s4 svjae

1

Phenylhydrazine-Induced Anemia in Mice

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129/SvImJ (wild-type), 129S4/SvJae (wild-type), or 129S4/SvJae-Pparαtm1Gonz/J female mice (The Jackson Laboratory) 6 – 8 weeks of age, randomized by weight, were pretreated with GW7647 (100 μg/kg) for 3 days (days −3 – −1). On day 0, the mice were injected with phenylhydrazine (PHZ) (60 mg/kg). GW7647 injection was continued during days 0 – 6. Whole blood samples were collected at each day of day 1 – 5, and day 7 and 9 for Complete Blood Count (CBC) analyses.
4 to 6 week old Nan/+ mutant mice (officially designated as Klf1Nan; http://www.informatics.jax.org/allele/MGI:1861107), obtained from the Jackson Laboratory, were injected with GW7647 (100 μg/kg) for 18 days. Mice were randomized by weight. Whole blood samples were collected every 3 days for CBC analyses.
All mouse procedures were approved by the Animal Care and Use Committees of the Massachusetts Institute of Technology (Cambridge, MA).
Lee H.Y., Gao X., Barrasa M.I., Li H., Elmes R.R., Peters L.L, & Lodish H.F. (2015). PPARα and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal. Nature, 522(7557), 474-477.
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2

Murine Studies of VWF-FVIII Clearance

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VWF−/− and FVIII−/− mice on C57BL/6J and 129S4/SvJae backgrounds, respectively were obtained from the Jackson Laboratory (Sacremento, CA). These mice were crossbred to generate VWF−/−FVIII−/− double-knockout mice and bred for 10 generations to C57BL/6J background. MGL1−/− mice were also obtained from the Jackson Laboratory. Mice were fed ad libitum with standard chow (LabDiet, IPS United Kingdom). In keeping with previous murine studies of VWF-FVIII clearance, studies were performed in both male and female mice,19 (link)–24 (link) and data combined in order to improve power. Consequently, potential sex differences were not investigated in the study. All in vivo studies were performed in accordance with the Health Product Regulatory Authority, Ireland (AE19127/P060) and approved by the Animal Research Ethics Committee of The Royal College of Surgeons in Ireland and Trinity College Dublin (REC1585 and 260219, respectively). Where indicated, clearance studies were repeated in the presence or absence of (1) clodronate-induced macrophage depletion16 (link),17 (link) or (2) polyclonal goat anti-mouse MGL1/2 antibody (R&D systems, Minneapolis) as previously described.16 (link),17 (link),20 (link) Plasma FVIII:C levels were measured using a chromogenic assay (HyphenBiomed, France).
Ward S.E., Guest T., Byrne C., Lopes P., O’Sullivan J.M., Doherty D., O’Connell D., Gutierrez Llaneza S., Chion A., Fazavana J., Fallon P.G., Preston R.J., Johnsen J.M., Pipe S.W., Turecek P.L, & O’Donnell J.S. (2023). Macrophage Galactose Lectin Contributes to the Regulation of FVIII (Factor VIII) Clearance in Mice—Brief Report. Arteriosclerosis, Thrombosis, and Vascular Biology, 43(4), 540-546.
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3

Geminin Conditional Knockout in Mice

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Geminin flox/flox (Gmnnfl/fl) mice (Shinnick et al., 2010 (link)) were maintained with a mixed genomic background of 129S4/SvJae and C57BL/6J (016913; Jackson Laboratory, Bar Harbor, ME), and Gdf9-cre mice (Lan et al., 2004 (link)) were maintained with C57/BL6 genomic background. Mutant mice were homozygous for the geminin floxed allele and heterozygous for Gdf9-cre (Gdf9-cre Gmnnfl/fl), and control mice were homozygous for Geminin floxed allele and Gdf9-cre negative (Gmnnfl/fl). Mice were housed in 12-h alternating light/dark cycles, with free access to water and food.
All experiments were conducted with the approval of the Animal Research Committee of the Institute of Zoology, Chinese Academy of Sciences. Mice were killed under standard protocols, and all efforts were made to minimize suffering.
Ma X.S., Lin F., Wang Z.W., Hu M.W., Huang L., Meng T.G., Jiang Z.Z., Schatten H., Wang Z.B, & Sun Q.Y. (2016). Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility. Molecular Biology of the Cell, 27(5), 768-775.
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4

Phenylhydrazine-Induced Anemia in Mice

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129/SvImJ (wild-type), 129S4/SvJae (wild-type), or 129S4/SvJae-Pparαtm1Gonz/J female mice (The Jackson Laboratory) 6 – 8 weeks of age, randomized by weight, were pretreated with GW7647 (100 μg/kg) for 3 days (days −3 – −1). On day 0, the mice were injected with phenylhydrazine (PHZ) (60 mg/kg). GW7647 injection was continued during days 0 – 6. Whole blood samples were collected at each day of day 1 – 5, and day 7 and 9 for Complete Blood Count (CBC) analyses.
4 to 6 week old Nan/+ mutant mice (officially designated as Klf1Nan; http://www.informatics.jax.org/allele/MGI:1861107), obtained from the Jackson Laboratory, were injected with GW7647 (100 μg/kg) for 18 days. Mice were randomized by weight. Whole blood samples were collected every 3 days for CBC analyses.
All mouse procedures were approved by the Animal Care and Use Committees of the Massachusetts Institute of Technology (Cambridge, MA).
Lee H.Y., Gao X., Barrasa M.I., Li H., Elmes R.R., Peters L.L, & Lodish H.F. (2015). PPARα and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal. Nature, 522(7557), 474-477.
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