The following specific Abs were used (polyclonal unless indicated): monoclonal FLAG (clone M2, 1:1,000, Sigma‐Aldrich), Myo7a (25‐6790, 1:400, Proteus), and mDia1(aa 66–77) (DP4471, 1:500, ECM Biosciences), whose immunogen is 100% conserved between mouse Dia1 and human DIA1. HRP‐conjugated Abs against GST (PM013‐7, 1:5,000), α‐tubulin (PM054‐7, 1:2,000), GAPDH (M171‐7, 1:2,000), and GFP (598–7, 1:2,000) were from MBL International. Alexa568‐conjugated phalloidin (1:500) and Alexa488‐conjugated secondary Abs (1:2,000) were from Invitrogen.
Monoclonal flag clone m2
Manufactured by Merck Group
Monoclonal FLAG (clone M2) is a laboratory reagent used for the detection and purification of recombinant proteins containing the FLAG tag. The FLAG tag is a short peptide sequence that can be attached to proteins to facilitate their identification and isolation. The Monoclonal FLAG (clone M2) antibody specifically recognizes and binds to the FLAG tag, enabling researchers to detect and purify the tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Alexa 568 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
Cyclopiazonic acid, by Merck Group (1 mentions)
Dantrolene, by Merck Group (1 mentions)
Valdecoxib, by Merck Group (1 mentions)
Glutamate, by Merck Group (1 mentions)
Celecoxib, by Merck Group (1 mentions)
Xestospongin c, by Merck Group (1 mentions)
2 5 dimethyl celecoxib, by Merck Group (1 mentions)
Celltiter glo luminescent cell viability assay atp, by Promega (1 mentions)
Brefeldin a, by Merck Group (1 mentions)
Caffeine, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
A23187, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Thapsigargin, by Merck Group (1 mentions)
Tunicamycin, by Merck Group (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (1 mentions)
2 protocols using monoclonal flag clone m2
1
Immunofluorescence Antibody Labeling Protocol
2
Antibody and Chemical Reagents in Cell Studies
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The antibodies used were monoclonal GFP (Roche Applied Science, Indianapolis, IN), monoclonal actin (clone C4; Abcam, Cambridge, MA), polyclonal actin (Sigma-Aldrich), polyclonal Gaussia luciferase (New England Biolabs), polyclonal BiP (Cell Signaling, Danvers, MA), monoclonal FLAG (clone M2; Sigma-Aldrich), and monoclonal Myc (clone 4A6; Millipore). Chemicals used (with diluent in parentheses) were thapsigargin (dimethyl sulfoxide [DMSO] in in vitro studies, ethanol in in vivo studies; Sigma-Aldrich), cycloheximide (DMSO; Sigma-Aldrich), brefeldin A (ethanol; Sigma-Aldrich), dithiothreitol (water; Sigma-Aldrich), tunicamycin (DMSO; Sigma-Aldrich), cyclopiazonic acid (DMSO; Sigma-Aldrich), A23187 (DMSO; Sigma-Aldrich), TPEN (DMSO; Sigma-Aldrich), dantrolene (DMSO; Sigma-Aldrich), xestospongin C (DMSO; Sigma-Aldrich), caffeine (water; Sigma-Aldrich), glutamate (1 N HCl; Sigma-Aldrich), celecoxib (DMSO; Sigma-Aldrich), valdecoxib (DMSO; Sigma-Aldrich), and 2,5-dimethyl-celecoxib (DMSO; Sigma-Aldrich). Vehicle controls at concentrations equivalent to treatments were used in all experiments. Cell viability assays were performed with the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) or the CellTiter-Glo Luminescent Cell Viability Assay (ATP) following the manufacturer's instructions (Promega, Madison, WI).
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