Trc 50 9 camera

Thirty-five–degree color fundus photographs centered on the fovea were obtained using a Topcon TRC 50IX camera or Topcon DRI Triton camera (Topcon Corp., Tokyo, Japan). OCT images were captured using a Spectralis HRA+OCT device (Heidelberg Engineering). In 94.5% of the individuals (2578/2727), grading of the images was performed according to the standard protocol of the Cologne Image Reading Center and Laboratory (CIRCL).²¹ (link) In 4.5% of the individuals (149/2727), images were graded according to the Wisconsin Age-Related Maculopathy Grading System and reclassified into the Rotterdam Classification (RC),22 (link), 23 (link), 24 (link) as described previously. This was the case if CIRCL grading (of the most recent image date) was not available. Individuals were categorized into 3 phenotype groups: no AMD (no signs of AMD or ≤10 small drusen ≤63 μm together with pigmentary changes [CIRCL]; RC grade 0), early or intermediate AMD (≥10 small drusen ≤63 μm together with pigmentary changes, ≥1 intermediate drusen 63–124 μm, or large drusen ≥25 μm [CIRCL]; RC grade 1–3), advanced AMD (choroidal neovascularization [CNV] or geographic atrophy (GA) in at least 1 eye [CIRCL]; RC grade 4).

Standard ophthalmic imaging was performed to enable comparison of features imaged with the 1.7-MHz sweep rate OCTA system and findings visualized using classic diagnostics methods. Color fundus photographs and FA images were taken with a Topcon (TRC-50IX) camera, and ICGA images were collected with the Spectralis HRA + OCT system. In addition, imaging was performed with AngioPlex Cirrus HD-OCT Angiography apparatus (Carl Zeiss Meditec, Inc., California, USA) a spectral-domain OCT (SDOCT) system with 840-nm center wavelength.⁴⁵ The imaging with $5\text{-}\mu \mathrm{m}$ axial resolution at full width at half maximum (FWHM), and $15\text{-}\mu \mathrm{m}$
$1/e^2$ beam spot diameter at the retina was performed at 68-kHz A-scan rate. The scanning protocols and the parameters of vascular layer visualization are presented in Tables 1 and 2. The OCTA method implemented in the AngioPlex Cirrus HD-OCT instrument is OMAG. All images from the latter system were generated by the AngioPlex software.

Following pupillary dilation, fundus photographs were taken of unanesthetized mice using appropriate filters using the TRC 50-IX camera (Topcon, Japan) integrated with the OphthaVision digital imaging suite (Sonomed Escalon). Images were exported from the Topcon imaging suite as TIFF files and prepared using Adobe Photoshop (Adobe, San Jose, CA, USA).