Proteominer low abundance protein enrichment kit

Nutmeg seeds were purchased from Shenyang Medicinal Materials Market (Shenyang, China), the seeds were ground into fine powder and stored at 4 °C in a sealed plastic bag. Nutmeg powder was suspended in normal saline before each time use. MDA ELISA, MAO ELISA, Glutathione peroxidase (GSH-Px) ELISA, Glutathione S-transferase (GSTs) ELISA kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China). Tandem Mass Tagging (TMT) Kits and Reagents was purchased from Thermo Fisher (Waltham, MA, USA), Bradford protein quantification kit was purchased from Beyotime (Shanghai, China). Dithiothreitol (DTT), triethyl ammonium bicarbonate buffer solution (TEAB), iodoacetamide (IAM), ammonia water and ammonium bicarbonate were purchased from Sigma (St. Louis, MO, USA). Sodium dodecyl sulfate (SDS) and urea were purchased from Sinopharm (Shanghai, China). Mass spectrometry grade pancreatin was purchased from Promega (Madison, WI, USA). Liquid chromatography-Mass spectrometry (LC-MS) grade ultrapure water, LC-MS grade acetonitrile and LC-MS grade formic acid were purchased from Thermo Fisher Chemical (Waltham, MA, USA). Acetone was purchased from Beijing Chemical Works Co., Ltd. (Beijing, China). ProteoMiner Low Abundance Protein Enrichment Kit was purchased from Bio-Rad (Hercules, CA, USA). LC-MS/MS analysis was performed by Novogene Co., Ltd. (Beijing, China).