Human igfbp7 elisa kit

Tissue metalloproteinase 2 (TIMP2) and insulin-like growth factor binding protein-7 (IGFBP7) were measured in urine using the human TIMP-2 Quantikine ELISA kit (Biotechne) and human IGFBP7 ELISA kit (abcam). Neutrophil gelatinase-associated lipocalin (NGAL) was measured in urine using Human Lipocalin-2/NGAL Quantikine ELISA Kit (BioTechne), Serum troponin was measured using High-Sensitivity Troponin I kit on Advia Centaur XP. Serum NT-proBNP was measured using NT-proBNP human ProcartaPlex™ Simplex kit (ThermoFisher) on the MAGPIX platform (Luminex).

The urine samples were collected on admission to critical care areas (day 1). The urine was frozen at −80 °C within the first 30 min after sample collection. The urinary concentrations of TIMP-2 and IGFBP7 were determined using commercially available ELISA kits (Human TIMP-2 Quantikine ELISA Kit, R&D, Minneapolis, Minnesota; Human IGFBP7 ELISA Kit, Abcam, Cambridge, UK) following manual instructions. The ELISA plates were read at O.D. of 450 and the calculations were done according to the signal that was given by the standard curve of each kit. NGAL determinations were done using the NGAL kit (Abbott, Chicago, IL, USA) according to the manual instructions and using the Abbott™ ARCHITECT™ Analyzer.

The urine samples were collected on day 1 and on day 5. The urine was frozen at −80 °C within the first 30 min after sample collection. The urinary concentrations of TIMP-2 and IGFBP7 were determined using commercially available ELISA kits (Human TIMP-2 Quantikine ELISA Kit, R&D, Minneapolis, Minnesota, USA; Human IGFBP7 ELISA Kit, Abcam, Cambridge, UK) following manual instructions. The ELISA plates were read at O.D. of 450 and the calculations were done according to the signal that was given by the standard curve of each kit. NGAL determinations were done using the NGAL kit (Abbott, Chicago, IL, USA) according to the manual instructions and using the Abbott ™ ARCHITECT™ Analyzer (Chicago, IL, USA).