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Mln4924

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MLN4924 is a small molecule inhibitor that targets the NEDD8-activating enzyme (NAE). It is used in research applications to study the role of the NEDD8 pathway in cellular processes.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Fujifilm (3 mentions) Pomalidomide, by Merck Group (3 mentions) Mg132, by Peptide Institute (3 mentions) Lenalidomide, by Fujifilm (3 mentions) Thalidomide, by Tokyo Chemical Industry (2 mentions) 5 hydroxythalidomide, by Peptide Institute (2 mentions) Dbet1, by MedChemExpress (1 mentions) Arv825, by MedChemExpress (1 mentions) Thymidine, by Merck Group (1 mentions) Lovastatin, by Merck Group (1 mentions) Aphidicolin, by Merck Group (1 mentions) Centrinone, by MedChemExpress (1 mentions) Mg132, by Fujifilm (1 mentions) Nocodazole, by Fujifilm (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) 2 3 cgamp, by InvivoGen (1 mentions) Z vad fmk, by Peptide Institute (1 mentions) Recombinant human tnf α, by R&D Systems (1 mentions) Thalidomide, by Merck Group (1 mentions)

4 protocols using mln4924

1

Dissolution and Dilution of Therapeutic Compounds

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Thalidomide (Tokyo Chemical Industry), pomalidomide (Sigma-Aldrich), lenalidomide (FUJIFILM Wako), 5-hydroxyThalidomide (prepared according to a previously published method64 (link)), dBET1 (MedChemExpress), ARV-825 (MedChemExpress), indisulam (BLD Pharmatech), MG132 (Peptide Institute), and MLN4924 (Chemscene) were dissolved in DMSO (FUJIFILM Wako) at 2–100 mM and stored at −20 °C as stock solutions. All drugs were diluted 1000-fold for in vivo experiments or 200-fold for in vitro experiments.
Yamanaka S., Horiuchi Y., Matsuoka S., Kido K., Nishino K., Maeno M., Shibata N., Kosako H, & Sawasaki T. (2022). A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues. Nature Communications, 13, 183.
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2

Cellular Checkpoint Modulation Assay

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The following chemicals were used: Centrinone (MedChem Express, HY-18682), MG132 (Wako, 135-18453), MLN4924 (Chemscene, CS-0348), Aphidicolin (Sigma, A0781), Lovastatin (Merck, 438185), Thymidine (Sigma, T1895), and Nocodazole (Wako, 140–08531).
Yamamoto S, & Kitagawa D. (2019). Self-organization of Plk4 regulates symmetry breaking in centriole duplication. Nature Communications, 10, 1810.
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3

Compound Preparation and Handling for Research

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Thalidomide (Tokyo Chemical Industry), pomalidomide (Sigma–Aldrich), lenalidomide (FujiFilm Wako), 5-hydroxyThalidomide (5-HT, synthesized as previously reported45 (link)), MG132 (Peptide Institute), MLN4924 (Chemscene), cycloheximide (Merck Millipore), and zVAD-FMK (Peptide Institute) were dissolved in DMSO (FujiFilm Wako) at 2–100 mM and stored at −20 °C as stock solutions. All drugs were diluted 1000-fold for in vivo experiments or 200-fold for in vitro experiments. 2′,3′-cGAMP (InvivoGen) was dissolved in endotoxin-free water. Recombinant human TNF-α (R&D Systems) was reconstituted according to the manufacturer’s protocol.
Yamanaka S., Shoya Y., Matsuoka S., Nishida-Fukuda H., Shibata N, & Sawasaki T. (2020). An IMiD-induced SALL4 degron system for selective degradation of target proteins. Communications Biology, 3, 515.
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4

Preparation of Drug Solutions

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Thalidomide (Sigma‐Aldrich and Tokyo Chemical Industry Co., Ltd), pomalidomide (Sigma‐Aldrich), lenalidomide (FUJIFILM Wako Pure Chemical), 5‐hydroxyThalidomide (5‐hydroxyThalidomide was prepared according to a previously published method (Yamamoto et al, 2009)), MG132 (Peptide Institute) and MLN4924 (Chemscene) were dissolved in DMSO (FUJIFILM Wako Pure Chemical) at 2–100 mM and stored at −20°C as stock solutions. All drugs were diluted 1,000‐ or 500‐fold for in vivo experiments, or diluted 200‐fold for in vitro experiments.
Yamanaka S., Murai H., Saito D., Abe G., Tokunaga E., Iwasaki T., Takahashi H., Takeda H., Suzuki T., Shibata N., Tamura K, & Sawasaki T. (2021). Thalidomide and its metabolite 5‐hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF. The EMBO Journal, 40(4), e105375.
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