Human GBM and normal brain tissues were obtained from the Cooperative Human Tissue Network (CHTN). Anti-FRMD6 (Sigma), -Lats1/2 (Bethyl Lab), -MST1/2, -YAP, -c-Met, -PDGFRA, -PDGFRB, and -merlin (Santa Cruz), -actin (Sigma), -v5 epitope (Invitrogen), -phospho-Lats1, -phospho-YAP (Cell signaling), -phospho-c-Met (Invitrogen and Santa Cruz), and -phospho-PDGFRA/B (Santa Cruz and R & D Systems) antibodies were used in the experiments. Anti-Ki67 was from the Fisher Scientific. Secondary anti-rabbit Alexa Fluor® 594 and anti-mouse Alexa Fluor® 488 were from Invitrogen.
Lats1 2
Manufactured by Fortis Life Sciences
Lats1/2 is a lab equipment product that serves as a core component in various biological research applications. It functions as a key regulatory protein involved in the Hippo signaling pathway, which plays a crucial role in cell growth, proliferation, and tissue homeostasis. The Lats1/2 product provides researchers with a tool to study these important biological processes without any extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Pdgfra, by Santa Cruz Biotechnology (1 mentions)
Phospho lats1, by Cell Signaling Technology (1 mentions)
Phospho yap, by Cell Signaling Technology (1 mentions)
Merlin, by Santa Cruz Biotechnology (1 mentions)
Pdgfrb, by Santa Cruz Biotechnology (1 mentions)
V5 epitope, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
C met, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
Pparg, by Proteintech (1 mentions)
Acsl4, by Proteintech (1 mentions)
Acsl1, by Proteintech (1 mentions)
Ppara, by Proteintech (1 mentions)
Eukitt mounting medium, by Avantor (1 mentions)
Rabbit anti yap1, by Cell Signaling Technology (1 mentions)
3 protocols using lats1 2
1
Immunofluorescence Analysis of GBM Markers
2
Western Blot Analysis of Ferroptosis Regulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed with RIPA (R0020, Solarbio, Beijing) containing Protease and Phosphatase Inhibitor Cocktail (P002, NCM Biotech, Suzhou) and collected for centrifugation. The steps in this laboratory are described in the previous article about Western experiments. Antibodies used are as follows: FAM60A (1:1,000, Novus, H00058516-B02P), GPX4 (1:1,000, Proteintech, 67763), ACSL1 (1:1,000, Proteintech, 13989), ACSL4 (1:1,000, Proteintech, 22401), PPARA (1:1,000, Proteintech, 15540), PPARG (1:1,000, Proteintech, 16643), YY1 (1:1,000, CST, #46395), MST1 (1:1,000, CST, #14946), p-MST1/2 (1:800, Abcam, ab79199), YAP (1:1,000, CST, #14074), p-YAP (1:700, CST, #13008), p-LATS1 (1:1,000, CST, #9157), LATS1/2 (1:1,000, Bethyl Laboratories, Montgomery, TX), and ACTB (1:1,000, Abways, AB0035).
3
Immunohistochemical Analysis of LATS2 and YAP1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue sections (3-μm thick) were prepared from formalin-fixed, paraffin-embedded human tissues and underwent standard immunohistochemistry protocols for LATS2 (rabbit anti-LATS1/2, 1:100, 2 h; Bethyl), YAP1 (rabbit anti-YAP1, 1:100, 1 h; Cell Signaling), or H pylori (rabbit anti–H pylori 1:100, 2 h, cat. B0471; DAKO) antibodies, and then with horseradish-peroxidase–labeled anti-rabbit EnVision System (30 min; DAKO).17 Immunolabeling was shown after a 10-minute incubation in liquid diaminobenzidine-chromogen substrate (cat. K3468; DAKO). Slides were counterstained with hematoxylin, dehydrated, and mounted with Eukitt-mounting medium (VWR). Relative quantification of the percentage of gastric epithelial cells expressing nuclear LATS2 and nuclear YAP1 was determined by a double-blind scoring using a scale from 1 to 4 (0, 0%; 1, <5%; 2, 5%–25%; 3, 25%–50%; and 4, >50%).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!