Anp32a

The following antibodies should be utilized for Western blot analysis. ANP32B (cat. No. ab200836; Abcam, USA), ANP32A (cat. No. 15810-1-AP; Proteintech, USA) and C-MYC (cat. No. 9402S; Cell Signal Technology, USA), PLK1 (cat. No. ab17056; Abcam, USA), Anti-acetyl-Histone H3 antibody (cat. No. 06-599; Millipore, USA), Histone H3 (D1H2) XP^® Rabbit mAb (cat. No. 4499S; Cell Signal Technology, USA), H3K27ac (cat. No. ab4729; Abcam, USA), Monoclonal ANTI-FLAG M2 antibody (Lot#SLBS3530V; Sigma, GER), CCNB1(cat. No. ab32053; Abcam, USA), CDK2 (cat. No. ab32147; Abcam, USA), BAX (cat. No. ab32503; Abcam, USA). The reference protein used was GAPDH (cat. No. MA3374; Millipore).

Immunoblotting was performed with primary antibodies against ANP32A (Abcam, Cambridge, United Kingdom), E-cadherin, Slug, Vimentin and Claudin-1 (Cell Signaling Technology, Danvers, MA, USA). GAPDH (Millipore, Billerica, MA, USA), HDAC1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as a loading control. The signal was visualized with super enhanced chemiluminescence (ECL) detection reagent (Millipore, Billerica, MA, USA).