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Mouse anti human fibronectin antibody

Manufactured by Merck Group
Sourced in United States

The Mouse anti-human fibronectin antibody is a laboratory reagent used for the detection and analysis of the extracellular matrix protein fibronectin. It is a monoclonal antibody that specifically binds to human fibronectin. This antibody can be utilized in various immunochemical techniques, such as ELISA, immunoblotting, and immunohistochemistry, to study the expression and distribution of fibronectin in biological samples.

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2 protocols using mouse anti human fibronectin antibody

1

Epithelial-Mesenchymal Transition Antibody Panel

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Mouse anti-human E-Cadherin antibody (for western blot) was purchased from BD Bioscience (San Jose, CA, USA). Rabbit anti-human ZO-1, Mouse anti-human α-SMA, FITC-conjugated anti mouse, and FITC-conjugated anti Rabbit antibodies were obtained from Invitrogen (Carlsbad, CA, USA). Rabbit anti-human ZEB1 antibody, Mouse anti-human fibronectin antibody was from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-human SLUG antibody, Rabbit anti-human E-Cadherin antibody (for immunofluorescence) and rabbit anti-human β-actin antibody was purchased from Abcam (Cambridge, MA, USA). Rabbit anti-human Snail antibody was from Santa-Cruze Biotechnology (Santa Cruz, CA, USA). Mouse anti-human Smad2/3, p-Smad2/3, p38, and p-p38 antibodies were from Cell Signaling Technology (Danvers, MA, USA). TRIzol reagent and DAPI (4’,6-diamidino-2-phenylindole) were bought from Invitrogen (Carlsbad, CA, USA). SuperReal PreMix Plus (SYBR Green) reagent kit was from Takara Clontech (Kyoto, Japan). Most of other reagents such as salt and buffer components were analytical grade and obtained from Sigma-Aldrich (St. Louis, MO, USA).
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2

Immunostaining of Cell-Cell Junctions

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Mouse anti-human E-cadherin antibody (category number: 610182) was purchased from BD Biosciences (San Jose, CA, USA). Rabbit anti-human ZO-1 (category number: 61-7300), Alexa Fluor 594-conjugated anti-rabbit antibody, and FITCconjugated anti-mouse antibody were obtained from Invitrogen (Carlsbad, CA, USA). Mouse anti-human fibronectin antibody was purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Mouse anti-human connexin 43 antibody (category number: ab11370) and rabbit anti-human b-actin antibody were purchased from Abcam (Cambridge, MA, USA). TRIzol reagent and 4 0 ,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen. The SuperReal PreMix Plus (SYBR Green) reagent kit was obtained from Takara Clontech (Kyoto, Japan). Most of the other reagents, such as salt and buffer components, were of analytical grade and were obtained from Sigma-Aldrich Corp.
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