To verify the purity of the purified enzymes, a UV/Vis spectrum of each enzyme was recorded from 250 to 600 nm in the oxidized and reduced state using an Agilent 8543 diode array spectrometer (Agilent Technologies, Santa Clara, California, USA). Purified CDH was diluted in 50 mM sodium acetate pH 5.5 (sparged with N2 gas for at least 30 min) to an absorbance of approximately 1.1 at 280 nm, and separated into two cuvettes. To measure the reduced form of the enzyme, cellobiose was added to a final concentration of 1 mM (~1000‐fold molar excess), while for the oxidized form the same amount of water was added.
Agilent 8543
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 8543 is a high-performance, versatile laboratory instrument designed for a wide range of analytical applications. It is a compact, modular system that can be configured with various modules to meet specific testing requirements. The core function of the Agilent 8543 is to provide accurate and reliable measurements, but its intended use is not specified in this description.
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Lab products found in correlation
2 protocols using agilent 8543
1
Spectroscopic Characterization of Purified Enzymes
2
Characterization of Novel Surfactant Compounds
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All materials and reagents used in this study were analytical grade obtained from Sigma Aldrich and used as its. The Stuart SMP30 (115/230 VAC, integrated cooling 350 °C to 50 °C in 10 min, United Kingdom) melting point instrument was used to determine the melting points of NSB and NCSB. FTIR spectra of NSB and NCSB were obtained using an FT/IR-6300 Fourier Transform Infrared Spectrometer (Jasco, Japan). A Bruker NMR instrument was used to record 1 H NMR spectra at 400 MHz of NSB and NCSB.
The NCSB sensor's mass spectra were recorded using an LC-MS Spectrometer Model Q-ToF Micro Waters. The fluorescence experiments were carried out on a Jasco FP-6500 spectrometer with a quartz cuvette recorded at 400 MHz using a Bruker NMR instrument. The Mass spectra of the surfactant were recorded on MS Spectrometer Model Q-ToF Micro Waters. The fluorescence experiments were performed on an FP-6500 spectrofluorometer (Jasco, Japan) using a quartz cuvette with a 1-cm path length. The absorption spectra were obtained using an Agilent 8543 (Agilent, USA) UV/Vis spectrophotometer.
The NCSB sensor's mass spectra were recorded using an LC-MS Spectrometer Model Q-ToF Micro Waters. The fluorescence experiments were carried out on a Jasco FP-6500 spectrometer with a quartz cuvette recorded at 400 MHz using a Bruker NMR instrument. The Mass spectra of the surfactant were recorded on MS Spectrometer Model Q-ToF Micro Waters. The fluorescence experiments were performed on an FP-6500 spectrofluorometer (Jasco, Japan) using a quartz cuvette with a 1-cm path length. The absorption spectra were obtained using an Agilent 8543 (Agilent, USA) UV/Vis spectrophotometer.
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