Growth curves were generated to determine the rate of proliferation in the exponentially growing young and old fibroblasts. Cells were plated in six-well plates with 50,000 cells/dish. Cell numbers were counted days 1–5 (using Beckman Z1 particle counter; Beckman Coulter, Fullerton, California) and the growth curves (cell#/time) were constructed. The doubling time (DT) was calculated from the exponential part of the growth curve using the equation: DT = 0.693*t/ ln (Nt/N0) where t is the time in hours, Nt is number of cells at time t and N0 is the number of cells at the initial time. The average DT is calculated with the doubling times from passages 3–7.
Beckman z1 particle counter
Manufactured by Beckman Coulter
The Beckman Z1 particle counter is a laboratory instrument used to measure and analyze the size and concentration of particles in a liquid or gas sample. It provides accurate and reliable data on particle characteristics without interpretation or extrapolation.
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Lab products found in correlation
3 protocols using beckman z1 particle counter
1
Fibroblast Growth Curve Analysis
2
Platelet Count Quantification Protocol
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Cell counts from platelet poor plasma, platelet rich plasma, and platelets in measurement buffer were acquired using a Beckman Z1 Particle Counter (Beckman Coulter, Brea, CA). Size restrictions were set to measure particles between 1.8 and 3.9 µm and measurements were done according to the manufacturer’s protocol.
3
Glioma Cell Proliferation Assay
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Glioma cells were seeded in 96-well plate with a concentration of 3000 cells per well, and cultured at 37 °C. The cell number was measured by the Beckman Z1 particle counter (Beckman Coulter). The absorbance in each well was measured at 490 nm with a microplate reader and cell proliferation curves were plotted against time.
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