Poly dl lactic co glycolic acid

Poly (DL-lactic-co-glycolic) acid (85:15, ester-terminated, 0.55 – 0.75 dL/g) was from Durect Corporation (Birmingham, AL). PEG2000-hydrazone-C18 (PHC), PEG2000-amide-C18 (PAC), and O-stearoyl mannose (M-C18) were synthesized using methods previously published by our laboratory ^{20 (link)}. BA and prednisolone were from TCI America (Portland, OR). Cyanine7.5 NHS ester (Cy7.5) was from Lumiprobe (Hallandale Beach, FL). TNF-α ELISA kit was from Thermo-Fisher Scientific (Waltham, MA). LPS and dimethyl-9,9′-biacridinium dinitrate (lucigenin) were from Sigma-Aldrich (St. Louis, MO). All solvents were of HPLC grade and from Sigma Aldrich.
Mouse J774A.1 macrophage cells were from the American Type Culture Collection (Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% v/v fetal bovine serum (FBS) and penicillin/streptomycin (100 U/mL), all from Gibco (Grand Island, NY).
All female C57BL/6 mice (18–20 grams) were from Charles River Laboratories (Wilmington, MA). Animal studies were conducted following the U.S. National Research Council Guidelines for Care and Use of Laboratory Animals. The animal protocol was approved by the Institutional Animal Care and Use Committee at The University of Texas at Austin.

Poly (DL lactic-co-glycolic acid), 50:50 with inherent viscosity 0.95–1.20 dL/g, was purchased from DURECT Corporation (Birmingham, AL). Poly(beta amino ester) (PBAE) was synthesized by a Michael addition reaction of 1,4-butanediol diacrylate (Alfa Aesar Organics, Ward Hill, MA) and 4,4′- trimethylenedipiperidine (Sigma, Milwaukee, WI) as previously reported.^{[15 ]} DSPE-PEG(2000)-maleimide was purchased from Avanti Polar Lipids (Alabaster, AL). MPG peptide (GALFLGFLGAAGSTMGAWSQPKKKRKV) was purchased from the W.M. Keck Facility (Yale University). The donor DNA oligos were purchased from Keck (Yale University). Donor DNA was 5’ and 3’ end protected by three phosphorothioate internucleoside linkages. PNA monomers were purchased from ASM research chemicals and PNA was synthesized by standard Boc based protocols.^{[28 (link)]} In the PNA sequence, ‘O’ represents the 8-amino-2,6-dioxaoctanoic acid linker and ‘J’ stands for pseudoisocytosine, a replacement for cytosine that allows pH-independent triplex formation. Donor DNA was purchased from Midland Certified Reagent Company (Midland, TX, USA). The tcPNA sequence is: N terminus-KKK-JTTTJTTTJTJT-OOO-TCTCTTTCTTTCAGGGCA-KKK-C terminus. The Donor DNA sequence is: 5’-AAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATCTCTGCATATAAATAT-3’

Poly(D, L lactic-co-glycolic acid), 50:50 was purchased from DURECT Corporation (Brimingham). Poly(beta amino ester) (PBAE) was synthesized by a Michael addition reaction of 1,4-butanediol diacrylate (Alfa Aesar Organics, Ward Hill) and 4,4′-trimethylenedipiperidine (Sigma, Milwaukee) as previously reported(24 (link)). The backbone plasmid pGL4.17[luc2/Neo] was purchased from Promega.