Log In Sign up
The largest database of trusted experimental protocols

Tocriscreen plus

Manufactured by Bio-Techne
Visit Supplier
Sourced in Sweden

The Tocriscreen Plus is a comprehensive screening library developed by Tocris Bioscience, a brand of Bio-Techne. It contains a diverse collection of biologically active compounds that can be used for cell-based screening and target identification applications.

Automatically generated - may contain errors

Lab products found in correlation

Lopac1280, by Merck Group (2 mentions) Trans isrib, by Bio-Techne (2 mentions) Chroman 1, by MedChemExpress (2 mentions) Polyamine supplement, by Merck Group (2 mentions) Antioxidant supplement, by Merck Group (2 mentions) Emricasan, by Selleck Chemicals (2 mentions) Y 27632, by Bio-Techne (2 mentions)

3 protocols using tocriscreen plus

1

Diverse Chemical Library Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
Compounds were selected from several chemical libraries: Acids (Vitas-M Laboratory); Consortium set (Specs)*; Drug Like (Enamine)*; Hit Finder (Maybridge)*; Ion Channel Ligand and Nuclear Receptor Ligand (Enzo Life Sciences); Known Drugs (Prestwick Chemical); LCBKI Primary Screening Set (Chemical Biology Consortium Sweden)*; Natural derivatives (TimTec); NIH Clinical Collection (NIH); Peptidomimetics (ChemDiv Inc.)*; and Tocriscreen Plus (Tocris Bioscience). Apart from LCBKI, provided by the Chemical Biology Consortium Sweden, and the NIH clinical collection, provided by the Libraries Roadmap Initiative, all compound libraries were commercially available. Whenever a selection was made (*), a chemist was instructed to search for small, hydrophobic, and partially charged compounds.
Liin S.I., Lund P.E., Larsson J.E., Brask J., Wallner B, & Elinder F. (2018). Biaryl sulfonamide motifs up- or down-regulate ion channel activity by activating voltage sensors. The Journal of General Physiology, 150(8), 1215-1230.
+ Open protocol
+ Expand
2

High-Throughput Screening of CEPT Cocktail

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ROCK inhibitor Y-27632 (#1254) and Trans-ISRIB (#5284) were purchased from Tocris. Chroman 1 (#HY-15392) was purchased from MedChem Express. Emricasan (#S7775) was purchased from Selleckchem. Polyamine supplement (#P8483) and antioxidant supplement (#A1345) were purchased from Sigma-Aldrich. To prepare the CEPT cocktail the following concentrations were used: 50 nM Chroman 1, 5 μM Emricasan, Polyamine supplement diluted 1:1000 according to manufacturer’s recommendation, 0.7 μM Trans-ISRIB. The small molecule libraries used for HTS include the NCATS Pharmacologically Active Chemical Toolbox (NPACT) designed to inform on novel phenotypes, biological pathways and cellular processes; the NCATS Pharmaceutical Collection (NPC) including FDA-approved drugs; the Mechanism Interrogation PlatE (MIPE); and the commercially available libraries Tocriscreen Plus (Tocris) and LOPAC 1280 (Sigma-Aldrich). More information and details on NCATS libraries are available here: https://ncats.nih.gov/preclinical/core/compound
Chen Y., Tristan C.A., Chen L., Jovanovic V.M., Malley C., Chu P.H., Ryu S., Deng T., Ormanoglu P., Tao D., Fang Y., Slamecka J., Hong H., LeClair C.A., Michael S., Austin C.P., Simeonov A, & Singeç I. (2021). A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. Nature methods, 18(5), 528-541.
+ Open protocol
+ Expand
3

High-Throughput Screening of CEPT Cocktail

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ROCK inhibitor Y-27632 (#1254) and Trans-ISRIB (#5284) were purchased from Tocris. Chroman 1 (#HY-15392) was purchased from MedChem Express. Emricasan (#S7775) was purchased from Selleckchem. Polyamine supplement (#P8483) and antioxidant supplement (#A1345) were purchased from Sigma-Aldrich. To prepare the CEPT cocktail the following concentrations were used: 50 nM Chroman 1, 5 μM Emricasan, Polyamine supplement diluted 1:1000 according to manufacturer’s recommendation, 0.7 μM Trans-ISRIB. The small molecule libraries used for HTS include the NCATS Pharmacologically Active Chemical Toolbox (NPACT) designed to inform on novel phenotypes, biological pathways and cellular processes; the NCATS Pharmaceutical Collection (NPC) including FDA-approved drugs; the Mechanism Interrogation PlatE (MIPE); and the commercially available libraries Tocriscreen Plus (Tocris) and LOPAC 1280 (Sigma-Aldrich). More information and details on NCATS libraries are available here: https://ncats.nih.gov/preclinical/core/compound
Chen Y., Tristan C.A., Chen L., Jovanovic V.M., Malley C., Chu P.H., Ryu S., Deng T., Ormanoglu P., Tao D., Fang Y., Slamecka J., Hong H., LeClair C.A., Michael S., Austin C.P., Simeonov A, & Singeç I. (2021). A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. Nature methods, 18(5), 528-541.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!