All fermentation experiments were performed in triplicates. For the statistical evaluation two-tailed unpaired t-test with Welch’s correction was used to analyse the data in GraphPad PRISM software (Version 9.4.1) and significance was set at p < 0.05 to the control (GraphPad Software, San Diego, CA, USA). HPLC data were analysed using the software ChemStation for LC systems from Agilent (Agilent, Santa Clara, CA, USA). Agilent’s MassHunter software (Agilent, Santa Clara, CA, USA) was used for analysing the HS-SPME-GC-MS data. IEC data were analysed by Clarity chromatography software (DataApex, Prague, Czech Republic).
Chemstation for lc systems
Manufactured by Agilent Technologies
Sourced in United States
ChemStation for LC systems is a software package designed for data acquisition, processing, and reporting for Agilent liquid chromatography (LC) instruments. It provides the core functionality required to control, acquire, and analyze data from Agilent LC systems.
Automatically generated - may contain errors
3 protocols using chemstation for lc systems
1
Fermentation Experiments with Statistical Analysis
2
HPLC Analysis of TAC and DIC
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The contents of TAC and DIC in all samples were determined using HPLC. The HPLC system (Agilent, 1260 series, Santa Clara, CA, USA) consisted of a quaternary pump (G1310A), degasser (G1322A), autosampler (G1329), column thermostat (CO-1000), UV detector (G1314A), and data processing software (Agilent Chem Station for LC systems, Santa Clara, CA, USA). TAC was analyzed with a C8 column (Thermo®, 250 mm × 4.6 mm, 5 μm) with water/isopropyl alcohol/tetrahydrofuran (6/2/2, v/v/v) as the mobile phase at a flow rate of 0.8 mL/min at 55 °C, and a detection wavelength of 220 nm. DIC was analyzed using an XB-C18 column (Ultimate®, 250 mm × 4.6 mm, 5 μm) with methanol/4%acetic acid (v/v) aqueous solution (7/3) as the mobile phase at a flow rate of 1.0 mL/min at 30 °C and a detection wavelength of 276 nm.
3
Analytical Methods for Fermentation Metabolites
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The HPLC data were analysed using the software ChemStation for LC systems from Agilent (Agilent, Santa Clara, CA, USA). The HS-SPME-GC-MS data analyses were carried out using Agilent’s MassHunter software (Agilent, Santa Clara, CA, USA).
The graphics were created with GraphPad Prism 5 (Graph Pad Software, La Jolla, CA, USA) and statistical analyses were employed in the form of a one-way analysis of variance (ANOVA) in combination with Bonferroni’s multiple comparison test. For statistical analysis, all fermentations were done in triplicate and the values for the metabolites are the means of these triplicates.
The graphics were created with GraphPad Prism 5 (Graph Pad Software, La Jolla, CA, USA) and statistical analyses were employed in the form of a one-way analysis of variance (ANOVA) in combination with Bonferroni’s multiple comparison test. For statistical analysis, all fermentations were done in triplicate and the values for the metabolites are the means of these triplicates.
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