After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
6 diamidino 2 phenylindole dapi
Manufactured by Biosharp
Sourced in China
6-diamidino-2-phenylindole (DAPI) is a fluorescent dye commonly used in molecular biology and cell biology applications. It is a DNA-binding agent that selectively binds to adenine-thymine (A-T) rich regions in DNA.
Automatically generated - may contain errors
3 protocols using 6 diamidino 2 phenylindole dapi
1
EdU Proliferation Assay in Cell Lines
2
Apoptosis Detection by TUNEL Assay
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The above treated cells were harvested and permeabilized by 0.1% Triton X-100 at room temperature for 15 min. Afterward, the abovementioned cells were subjected to the In Situ Cell Death Detection Kit (Roche, Switzerland) and all the experimental procedure was according to the manufacturer’s instruction. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei. Finally, the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
3
Pluronic P123-Mediated Bioactive Scaffold Fabrication
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Pluronic P123 (EO 20 PO 70 EO 20 , Mw = 5800) was obtained from Sigma (USA). Tetraethoxysilane (TEOS) was purchased from Nanjing Chemical Reagent (China). IGF-1 was purchased from R&D USA. High glucose Dulbecco's Modied Eagle Medium (DMEM) and trypsin solution with the concentration of 0.25% were bought from Hyclone (Logan, Utah, US). Fetal bovine serum (FBS) was bought from Sangon Biotech.
6-Diamidino-2-phenylindole (DAPI) was purchased from Biosharp.
6-Diamidino-2-phenylindole (DAPI) was purchased from Biosharp.
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