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4 protocols using terpinyl acetate

1

Essential Oil Composition Analysis

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1,8 cineole, linalool and linalyl acetate with a purity > 97%, terpinyl acetate (mixture of two isomers) with a purity > 95%; β-caryophyllene (mixture of two isomers) with a purity > 98.5%,decane (purity >99%) high purity silica gel 70–230 mesh and silver nitrate on silica gel (about 10% wt, % loading + 230 mesh) were from Sigma-Aldrich (Milan, Italy). HPLC and analytical grade solvents were from Carlo Erba Reagenti (Rodano, Italy). Commercially available standards were chosen to confirm component identification in the essential oil and to quantify the EO components on the basis of their response factor towards an internal standard (decane) considering the potential shift of FID towards the different classes of compounds [21 ]. Acyclovir, used as a positive control for HSV-2 antiviral activity was purchased from Sigma-Aldrich (Milan, Italy).
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2

Synthetic Blackcurrant Juice Composition

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Hexanal, nonanal, undecane,
α-pinene, camphene, β-pinene, myrcene, α-phellandrene,
δ-3-carene, α-terpinene, p-cymene, limonene, cis-β-ocimene, trans-β-ocimene,
γ-terpinene, terpinolene, eucalyptol, terpinen-4-ol, bornyl
acetate, terpinyl acetate, β-caryophyllene, n-nonane, neryl acetate, and a homologous series of n-alkanes (C9–C30) of analytical purity were purchased from
Sigma-Aldrich (St. Louis, MO). Glucose, fructose, and sucrose were
obtained from Merck (Darmstadt, Germany), citric acid was obtained
from J.T. Baker (Deventer, The Netherlands), and pectin was obtained
from Herbstreith & Fox KG (Neuenburg, Germany). Sodium chloride
(99% purity) was from Sigma-Aldrich. Methanol and acetone (HPLC grade)
were purchased from J.T. Baker. The aforementioned sugars, organic
acids, and pectin were employed to prepare a synthetic blackcurrant
juice containing no volatiles following the composition detailed in Food Composition and Nutrition Tables.25  
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3

Terpenoid Standards Characterization Protocol

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Terpenoid standards used in the study and their purity percentages as obtained from Sigma-Aldrich (UK) were: alloaromadendrene (-) (98%), camphene (95%), camphor (R) (95%), caryophyllene (-) (trans) (98.5%), 1,8-cineole, citral (95%), p-cymene (99%), geraniol (98%), geranyl acetate (97%), ledene (+) (95%), limonene (R) (+) (97%), linalool (±) (95%), linalyl acetate (97%), 6-methyl-5-hepten-2-one (96%), myrcene (90%), α-pinene (-) (99%), β-pinene (-) (99%), sabienene hydrate (97%), α-terpinene (85%), γ-terpinene (97%), terpinolene (95%), terpinen-4-ol (95%), α-terpineol (96%), terpinyl acetate (±) (90%) and verbenol (95%).
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4

Comprehensive Chemical Compound Acquisition

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Benzalkonium chloride (BKC), glycerol, parafluoro aniline (PFA), polyethylene glycol-400 (PEG-400), carvacrol, isopropyl alcohol, decanol, tri-isobutyl phosphate, terpinyl acetate, sodium fluorescein and agarose were purchased from Sigma-Aldrich (UK). Benzyl alcohol, citronellal, linalyl acetate, 1-bromohexane and methyl palmitate were purchased from Fischer Scientific, UK. Instant ocean salt was from Aquarium Systems (UK).
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