Polyclonal rabbit anti actin

The monoclonal YL1/2 rat anti-detyrosinated tubulin antibody (1:200) and the mouse monoclonal and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) antibodies (1:500) were obtained from Millipore. The mouse monoclonal anti-cyclin A, anti-prospero and anti-cyclin B antibodies were purchased from the Developmental Studies Hybridoma Bank (1:10). The mouse monoclonal anti-FLAG (1:10,000) antibody was obtained from Stratagene. The rabbit anti-cyclin B antibody (1:10,000) has been described before36 (link) and was kindly provided by David Glover (University of Cambridge, UK). Polyclonal rabbit anti-actin (1:4,000) antibodies were obtained from Santa Cruz, and the rat anti-deadpan antibody (1 μg ml⁻¹) was obtained from Abcam. The affinity-purified rabbit anti-Mad2 polyclonal antibody (1:500) was provided by David Sharp (Albert Einstein College of Medicine, New York). Goat secondary peroxidase-conjugated antibodies (1:5,000) were obtained from Jackson ImmunoResearch Laboratories and donkey Alexa Fluor-conjugated secondary antibodies (1:1,000) were obtained from Life Technologies. For western blotting, ECL reagent was purchased from Thermo Fisher Scientific. Some of the western blots were cropped and the original scans of the blots are provided in Supplementary Fig. 6.