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Anti cd38 pc5

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The Anti-CD38-PC5.5 is a fluorescent-labeled antibody that binds to the CD38 antigen, which is expressed on the surface of various cell types, including plasma cells and certain lymphoid cells. It is used in flow cytometry applications to detect and analyze CD38-positive cells.

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Lab products found in correlation

Anti cd4 pe, by Beckman Coulter (1 mentions) Kaluza 2, by Beckman Coulter (1 mentions) Anti cd3 aa750, by Beckman Coulter (1 mentions) Anti cd8 aa700, by Beckman Coulter (1 mentions) Anti cd279 pd 1 pc7, by Beckman Coulter (1 mentions) Anti hla dr pb, by Beckman Coulter (1 mentions) Anti cd14 ecd, by Beckman Coulter (1 mentions) 10 multicolor navios flow cytometer, by Beckman Coulter (1 mentions) Cd45 krome orange, by Beckman Coulter (1 mentions) Canto x, by BD (1 mentions) Anti cd3 bv605, by BD (1 mentions) Anti cd27 pe, by BD (1 mentions) Anti cd4 bv605, by BD (1 mentions) Influx cell sorter, by BD (1 mentions)

3 protocols using anti cd38 pc5

1

Comprehensive Immunophenotyping of ICU Patients

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Blood samples were collected within 48 hours of ICU admission (Days 1–2 sample) and 4 days thereafter (Days 4–6 sample). Fresh whole blood was stained with different combinations of the following conjugated monoclonal antibodies: anti–CD4-PE, anti–CD3-AA750, anti–CD8-AA700, anti–CD38-PC5.5 or isotype control, anti-CD279 (PD-1)-PC7 or isotype control, anti–HLA-DR-PB or isotype control, anti–CD14-ECD, and CD45-Krome Orange (Beckman Coulter). Acquisition was performed on a 10-multicolor Navios flow cytometer and analyzed with the Kaluza 2.1 software (Beckman Coulter). Gating strategies are depicted in Figure E2.
Hue S., Beldi-Ferchiou A., Bendib I., Surenaud M., Fourati S., Frapard T., Rivoal S., Razazi K., Carteaux G., Delfau-Larue M.H., Mekontso-Dessap A., Audureau E, & de Prost N. (2020). Uncontrolled Innate and Impaired Adaptive Immune Responses in Patients with COVID-19 Acute Respiratory Distress Syndrome. American Journal of Respiratory and Critical Care Medicine, 202(11), 1509-1519.
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2

B-cell Subset Immunophenotyping by Flow Cytometry

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Cells were counted, and 5 × 105 cells/tube were incubated on ice for 30 minutes with cocktail of different antibodies (anti-CD20-APC-Alexa Fluor 700, Anti-Human IgD-APC, anti-CD27-PC7, anti-CD38-PC5.5, all from Beckman Coulter, Inc.) then washed, fixed in 1%-PFA PBS and analyzed by FACS (Canto X, BD Biosciences). For each experiment, unstained cells as well as single color controls were analyzed to check for individual compensations between fluorochromes. FACS data were analyzed and graphed using KALUZA and FlowJo softwares. Among CD20+ B-cell subsets were defined as follows: naïve (IgD+CD27−), unswitched memory (IgD+CD27+), switched memory (IgD−CD27+) and plasmablasts (CD38++CD27++).
Ali M.F., Dasari H., Van Keulen V.P., Cornec D., Vasmatzis G., Peikert T, & Carmona E.M. (2017). Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways. Scientific Reports, 7, 3869.
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3

Multiparameter B Cell Immunophenotyping

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CD19+ cells were blocked with normal mouse serum (NMS) then stained with an antibody master mix to ensure consistent staining. The antibody staining cocktail was freshly prepared using optimized antibody concentrations consisting of anti-CD3-BV605 (Clone HIT3a; BD Biosciences 564712), anti-CD4-BV605 (Clone SK3; BD Biosciences 565998), anti-CD19-APC-Alexa700 (Clone J3-119; Beckman Coulter A78837), anti-CD20-Pacific Blue (Clone B9E9; Beckman Coulter A74777), anti-CD27-PE (Clone M- T271; BD Biosciences 557330), anti-CD38-PC5.5 (Clone LS198-4-3; Beckman Coulter A70205), anti-CD43-FITC (Clone DFT1; Beckman Coulter IM3264U), and Aqua LIVE/DEADTM viability stain. Stained cells were resuspended at a final concentration of 10 x 106 cells/mL, and memory B cells and B1 cells were sorted on a BD Influx cell sorter.
Ray M.E, & Rothstein T.L. (2023). Human VH4-34 antibodies derived from B1 cells are more frequently autoreactive than VH4-34 antibodies derived from memory cells. Frontiers in Immunology, 14, 1259827.
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