As described in Cops et al., blood samples were centrifuged (2000 rpm, 10 min) and plasma was preserved (−20 °C) for later analysis. Plasma samples were analyzed for bilirubin, creatinine, urea, cystatin C, aldosterone and C-reactive protein (CRP) using an automated analyzer (Cobas 8000 ISE module and Cobas 8000 c702 and c502 module, Roche Diagnostics, Germany)15 (link),39 (link)–41 (link). Urine samples were centrifuged (1500 rpm, 5 min) and preserved (−20 °C) for later analysis. Urine samples were analyzed for creatinine, urea and albumin using an automated analyzer (Cobas 8000 ISE module and Cobas 8000 c702 and c502 module, Roche Diagnostics, Germany). Urinary kidney injury molecule 1 (KIM-1) concentrations were determined using the rat TIM-1/KIM-1/HAVCR DuoSet ELISA kit (DY3689, R&D Systems, USA) according to the manufacturer’s instructions and all measurements were performed in duplicate15 (link). Creatinine clearance (ml/min/kg) was calculated as follows = [(urinary creatinine (mg/dl) × urinary volume (ml/24 h))/(plasma creatinine (mg/dl) × 1440 min) * 1000]/body weight15 (link),42 (link). Likewise, urea clearance was calculated.
Cobas 8000 ise module
Manufactured by Roche
Sourced in Germany
The Cobas 8000 ISE module is a diagnostic instrument manufactured by Roche. The core function of this module is to perform ion-selective electrode (ISE) analysis to measure the concentration of specific electrolytes in biological samples, such as sodium, potassium, and chloride. The module is designed to be integrated into the Cobas 8000 analytical system, a comprehensive solution for clinical chemistry and immunoassay testing.
Automatically generated - may contain errors
3 protocols using cobas 8000 ise module
1
Biomarker Profiling in Plasma and Urine
2
Comprehensive Plasma and Urine Biomarker Analysis
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Blood samples were centrifuged (2000 rpm, 10 min) and plasma was preserved (-20°C) for later analysis. Plasma samples were analyzed for triglycerides, creatinine, cystatin C, urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, C-reactive protein (CRP) and aldosterone using an automated analyzer (Cobas 8000 ISE module and Cobas 8000 c702 and c502 module, Roche diagnostics, Germany) (Erdem et al., 2000; Vaidya et al., 2010; Zhao et al., 2016) (Cops et al., 2018a; Cops et al., 2018b) . Urine samples were centrifuged (1500 rpm, 5 min) and preserved (-20°C) for later analysis. Urine samples were analyzed for creatinine, urea, and albumin using an automated analyzer (Cobas 8000 ISE module and Cobas 8000 c702 and c502 module, Roche diagnostics, Germany). Urinary kidney injury molecule 1 (KIM-1) concentrations were determined using the rat TIM-1/KIM-1/HAVCR DuoSet ELISA kit (DY3689, R&D Systems, USA) according to the manufacturer's instructions and all measurements were performed in duplicate (Cops et al., 2018a; Cops et al., 2018b) . Creatinine clearance was calculated from the following equation: creatinine clearance (ml/min/kg) = [(urinary creatinine (mg/dl) x urinary volume (ml/24h))/(plasma creatinine (mg/dl) x 1440 min)]/body weight (kg) (Rafiq et al., 2012) .
3
Comprehensive Blood Analysis Protocol
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Hemoglobin, hematocrit, platelet count, and WBC were analyzed on a Cell-Dyn Sapphire analyzer (Abbot Diagnostics, Abbot Park, IL). The Leucocount kit from BD (BD Biosciences, San Jose, CA) was used for residual WBC analysis, with a BD FACSCanto II cytometer. Plasma hemoglobin was analyzed with the HemoCue Plasma/Low Hb photometer (HemoCue AB, Angelholm, Sweden) to calculate hemolysis.
Lactate and blood gas analysis was performed on whole blood samples with an ABL 800 FLEX (Radiometer Medical ApS, Brøndshøj, Denmark).
Glucose, potassium, and Factor VIII were analyzed with the Cobas 8000 ISE module (Roche Diagnostics GmbH, Rotkreuz, Switzerland). FVIII and fibrinogen levels were analyzed with the STA-R Evolution/STA-R Max platform (Stago S.A.S., Asnieres-sur-Seine, Paris, France).
All plasma samples were prepared by centrifugation at 1850 G for 10 min in RT.
Lactate and blood gas analysis was performed on whole blood samples with an ABL 800 FLEX (Radiometer Medical ApS, Brøndshøj, Denmark).
Glucose, potassium, and Factor VIII were analyzed with the Cobas 8000 ISE module (Roche Diagnostics GmbH, Rotkreuz, Switzerland). FVIII and fibrinogen levels were analyzed with the STA-R Evolution/STA-R Max platform (Stago S.A.S., Asnieres-sur-Seine, Paris, France).
All plasma samples were prepared by centrifugation at 1850 G for 10 min in RT.
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