AR42J cells were cultured to the confluence of 40% in 6-well plates. MiR-181b mimics, MiR-181b mimic-negative control (NC), miR-181b inhibitor, or miR-181b inhibitor-NC (Invitrogen, Carlsbad, CA, USA) were mixed with Lipofectamine 2000 (Invitrogen), and then added into the culture medium. After transfection for 24 h, total RNA and proteins were extracted for qRT-PCR and western blotting.
Mir 181b inhibitor nc
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MiR-181b inhibitor-NC is a laboratory reagent designed to inhibit the activity of the miR-181b microRNA in experimental settings. This product is intended for research use only and its core function is to provide a means to study the biological roles and regulatory mechanisms of miR-181b in various cellular and molecular processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mir 181b inhibitor nc
1
Investigating miR-181b Regulation in AR42J Cells
2
Modulation of Pancreatic Acinar miR-181b
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Pancreatic acinar cells were cultured to 40% confluence. miR-181b mimics,
miR-181b mimic-negative control (NC), miR-181b inhibitor, and miR-181b
inhibitor-NC (Invitrogen) were individually added to Lipofectamine 2000
(Invitrogen) and the resulting mixture was added to the cell medium. Total RNA
and all proteins were obtained 24 h post-transfection and stored for later
quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot
analyses.
miR-181b mimic-negative control (NC), miR-181b inhibitor, and miR-181b
inhibitor-NC (Invitrogen) were individually added to Lipofectamine 2000
(Invitrogen) and the resulting mixture was added to the cell medium. Total RNA
and all proteins were obtained 24 h post-transfection and stored for later
quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot
analyses.
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