The largest database of trusted experimental protocols

Sucrose

Manufactured by Bio-Rad
Sourced in United States

Sucrose is a disaccharide that is commonly used in various laboratory applications. It is composed of glucose and fructose, and it serves as a source of energy and carbon for biological systems. Sucrose is widely utilized in biochemical and molecular biology experiments, often as a component in buffers, culture media, and other solutions.

Automatically generated - may contain errors

3 protocols using sucrose

1

Reagents and Chemicals for Cell Culture Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fetal Bovine Serum (FBS) and Phosphate Buffered Saline (PBS) were purchased from Hyclone™, ThermoFisher Scientific, Waltham, MA, USA. HEPES, EDTA, EGTA, TEMED, isopropanol, aprotonin, pepstatin, phenylmethanesulfonyl fluoride (PMSF), leupeptin, sodium fluoride (NaF), sodium orthovanadate (Na3VO4), diethyldithiocarbamate (DDC), doxorubicin, etoposide, fludarabine, 5-flouroUracil (5-FU), cisplatin, dimethyl-9,9’-biacridinium dinitrate (Lucigenin), N-acetyl-cysteine (NAC) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Venetoclax (VEN) was purchased from Medchem Express LLC. Streptomycin–penicillin and l-glutamine were purchased from Gibco. Coomassie Blue dye and bovine serum albumins (BSA) were purchased from ThermoFisher Scientific. Methanol and sodium dodecyl sulphate (SDS) were purchased from Merck, Kenilworth, NJ, USA. Sucrose and 30% acrylamide/bis solution were purchased from Bio-Rad. Goat anti-mouse and anti-rabbit IgG horseradish peroxidase conjugated secondary antibodies were purchased from Pierce, TX, USA. Protein A agarose beads were purchased from Santa Cruz, TX, USA. Chloromethyl-2-,7-dichlorofluorescin diacetate (DCFDA) and MitoSox™ were purchased from Molecular Probes, Thermo Fisher Scientific.
+ Open protocol
+ Expand
2

Mandarin Fish Protein Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mandarin fish (Siniperca chuaisi) were bought in a Yonghui supermarket (Hangzhou, China). Pepsin and bicinchoninic acid (BCA) protein assay kits were purchased from Sigma (St. Louis, MO, USA). Anti-rabbit IgG peroxidase-conjugated antibody produced in goat was purchased from Polila (Beijing, China). 3,3′,5,5′-Tetramethylbenzidine (TMB) was purchased from Aladdin (Shanghai, China). Formic acid (FA) and acetonitrile (ACN) (MS grade) were bought from Thermo Fisher Scientific (Waltham, MA, USA). D-glucose, maltose, sucrose and other chemicals were purchased from Bio-Rad in the best available purity grade.
+ Open protocol
+ Expand
3

Polyclonal Antibody Production against Venomous PLDs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyclonal antibodies against recombinant PLDs from L. intermedia, L. laeta, and L. gaucho venoms were produced in mice as described previously [47 ]. Five animals were immunized with each PLD, and the pools were used in the subsequent assays to avoid distortions from individual immunological responses. NaCl and KCl were purchased from Merck (Darmstadt, Germany). Tryptone and yeast extract were obtained from Himedia (Mumbai, India). Chloramphenicol and ampicillin were purchased from USB Corporation (Cleveland, OH, USA). SM (sphingomyelin egg, chicken) and LysoPC (l-α-lysophosphatidylcholine egg, chicken) were purchased from Avanti Polar Lipids, Inc. Alabaster, AL, USA. Coomassie Blue dye, Tris, and sucrose were obtained from Bio-Rad (Hercules, CA, USA). Xylazine and ketamine were purchased from Rhobifarma (Hortolândia, São Paulo, Brazil).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!