Il 5rα

Manufactured by BD

Sourced in United States

IL-5Rα is a receptor component that binds to the IL-5 cytokine, which is involved in the differentiation and activation of eosinophils. It plays a role in the immune response and inflammatory processes.

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Lab products found in correlation

Cd11b, by BD (2 mentions) Cd16 32, by BD (2 mentions) Siglec f, by BD (2 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Efluor 450, by Thermo Fisher Scientific (1 mentions) Cd45r b220, by BD (1 mentions) Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions) Fix perm, by Thermo Fisher Scientific (1 mentions) Annexin 5, by BD (1 mentions) Percp cy5, by Thermo Fisher Scientific (1 mentions) Influx flow cytometer, by BD (1 mentions) Facsaria 2, by BD (1 mentions) Sca 1, by BD (1 mentions)

3 protocols using il 5rα

Analytical Flow Cytometry of Immune Cells

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All analytical flow cytometry was performed on a BD LSR II flow cytometer. Dead cells were identified by DAPI (4’,6-diamidino-2-phenylindole) and excluded from analysis where appropriate. Primary antibodies specific for the following markers were directly conjugated to FITC, PE, APC, PerCP-Cy5.5, eFluor-780, eFluor-450, or PE-Cy7 and purchased from eBioscience (CD3, CD4, CD8α, CD11b, CD16/32, CD34, CD45.1, CD45.2, CD45R(B220), CD49b(DX5), FcεR1 and Gr1) or from BD Biosciences (c-kit, IL-5Rα, Siglec F, CCR3, and Annexin V). For intracellular staining, surface-labeled cells were fixed in isotonic 3% paraformaldehyde and incubated with affinity-purified anti-CF (Hamilton et al., 2008 (link)) in Fix & Perm (Invitrogen) according to product instructions. Staining was detected with highly cross-adsorbed donkey anti-rabbit-FITC (Jackson Immunochemicals, Stratech, UK). For cell sorting experiments, eosinophil populations were sorted on a BD Influx flow cytometer.

Matthews S.P., McMillan S.J., Colbert J.D., Lawrence R.A, & Watts C. (2016). Cystatin F Ensures Eosinophil Survival by Regulating Granule Biogenesis. Immunity, 44(4), 795-806.

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Nasal Polyp Immune Cell Profiling

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Cells from the digested nasal polyp single cell suspension were stained with mAbs against CD45, CD3, CD4, CCR3, CD27, CD38, CD138 (eBiosciences), IL-5Rα and CD20 (BD Biosciences, Franklin Lakes NJ) to identify plasma cells/plasmablasts, B cells, eosinophils and expression of the IL-5Rα. Plasma cells were defined as CD45⁺/CD3⁻/CD20⁻/CD27⁺/CD38⁺/CD138⁺, B cells were defined as CD45⁺/CD3⁻/CD20⁺, and eosinophils were defined as SSC^Hi/CD45⁺/CCR3⁺.
Blood from atopic donors without nasal polyposis or chronic rhinosinusitis was obtained and stained with CD45, CCR3 and IL-5Rα to determine the level of IL-5Rα expression by peripheral eosinophils.

Buchheit K.M., Dwyer D.F., Ordovas-Montanes J., Katz H.R., Lewis E., Vukovic M., Lai J., Bankova L., Bhattacharyya N., Shalek A.K., Barrett N.A., Boyce J.A, & Laidlaw T.M. (2020). IL-5Rα marks nasal polyp IgG4 and IgE-expressing cells in aspirin-exacerbated respiratory disease. The Journal of allergy and clinical immunology, 145(6), 1574-1584.

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Multiparameter Flow Cytometry Analysis

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The collected bone marrow cells, splenocytes and lung cells were suspended with staining buffer (PBS containing 3% FBS) and stained with fluorescent-conjugated antibodies specific for CCR3 (Biolegend, San Diego, CA, USA), SiglecF (BD Biosciences, Franklin Lakes, NJ, USA), Ly6G (Biolegend), CD11b (BD Biosciences), CD11c (BD Biosciences), Ly6C (BD Biosciences), CD3e (TONBO Biosciences, San Diego, CA, USA), CD4 (Biolegend), CD8 (TONBO Biosciences), CD14 (Biolegend), CD16/32 (BD Biosciences), CD19 (TONBO Biosciences), CD25 (TONBO Biosciences), CD44 (TONBO Biosciences), CD45.1 (Biolegend), CD45.2 (Biolegend), B220 (TONBO Biosciences), Gr-1 (Biolegend), Sca-1 (BD Biosciences), Il-5Rα (BD Biosciences), CD34 (BD Biosciences), and c-kit (Biolegend). The cells were sorted with a FACSAria II (BD Biosciences) and data were analyzed with FlowJo software (Tree Star, Ashland, OR, USA).

Sato Y., Kato H., Ebina-Shibuya R., Itoh-Nakadai A., Okuyama R, & Igarashi K. (2017). Bach2 Controls Homeostasis of Eosinophils by Restricting the Type-2 Helper Function of T Cells. The Tohoku journal of experimental medicine, 241(3).

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