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4 protocols using benzyl alcohol

1

Optical Projection Tomography of Limb Buds

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Limb buds were fixed with 4% PFA overnight and then embedded in 1% low-melting agarose (Lonza). Excess agarose around the limb was removed with a razor blade. After that, agarose containing the limb buds was attached to the swivel base with Loctite for optical projection tomography (OPT) scanning (Henkel). Limb buds were treated with 100% MeOH for 3 h, and samples cleared in a 1:2 solution of benzyl alcohol (Wako): benzyl benzoate (Wako) overnight. A 3D image was taken with the OPT scanner 3001 (Bioptonics) and visualized by Avizo software (Maxnet).
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2

Comprehensive Volatile Compound Analysis

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n-Alkanes (C8–C40) were purchased from J&K Scientific, Beijing, China. Benzaldehyde, benzyl alcohol, (Z)-3-hexenyl acetate, methyl salicylate, and 2-phenylethanol were purchased from Wako Pure Chemical Industries Ltd, Osaka, Japan. Ethyl n-decanoate, geraniol, 1-hexanol, (Z)-3-hexenol, phenylacetaldehyde, α-farnesene, (E)-nerolidol, linalool, linalool oxide, polyvinylpolypyrrolidone (PVPP), and XAD-2 were purchased from Sigma-Aldrich Company Ltd., Louis, MO, USA. geraniol was purchased from TCI Development Co., Ltd, Shanghai, China. The Quick RNA isolation kit was purchased from Huayueyang Biotechnology Co., Ltd., Beijing, China. The 2× SYBR Green Universal PCR Mastermix was purchased from Bio-Rad Laboratories, Richmond, CA, USA.
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3

Synthesis of Functionalized Gold Nanoparticles

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All chemicals were commercially available
and were used without further purification. Hydrogen tetrachloroaurate(III)
tetrahydrate, sodium borohydride, PVP (K30, average molecular weight:
∼40 000), benzyl alcohol, p-methylbenzyl
alcohol, p-methoxybenzyl alcohol, and potassium carbonate
were purchased from Wako Pure Chemical Industries. trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile
(DCTB) and p-(trifluoromethyl)benzyl alcohol were
purchased from Tokyo Chemical Industry. Deuterated benzyl alcohol
(C6H5CD2OH) was purchased from Sigma-Aldrich.
Deionized water used was Milli-Q grade (>18 MΩ cm).
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4

Solvent Selection and Characterization

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The following 30 solvents (96.0%–99.0% pure) were used without purification: linear alkanes with different carbon chain length (n = 6–16); aromatic hydrocarbons; linear aliphatic alcohols with different carbon chain length (n = 1–9); aromatic alcohols with different carbon chain length of the alkyl group (n = 0–3); acetate esters; unsaturated hydrocarbons; and branched alkanes. Hexane, octane, nonane, decane, dodecane, tetradecane, hexadecane, toluene, styrene, methanol, ethanol, 1-heptanol, 1-octanol, 1,4-butanediol, benzyl alcohol, 2-phenylethanol, propyl acetate, iso -butyl acetate, tert -butyl acetate, butyl acetate, pentyl acetate, octyl acetate, 1-octadecene, and squalene were supplied by Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Butylbenzene, tert-Butylbenzene, octylbenzene, 1-nonanol, and 3-phenyl-1-propanol were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Diluted ethane and ethylene (99.5% pure) and undiluted acetylene (0.1% in N2) were used as standard gases for gas chromatography. These gases were supplied by GL sciences Inc. (Tokyo, Japan). For the spin-trapping agent, we used 5,5-dimethyl-1-pyrroline N-oxide (DMPO; > 97.0% pure), which was supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
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