P vegfr2

Manufactured by Abcam

Sourced in United States

P-VEGFR2 is a laboratory reagent that detects the phosphorylated form of VEGFR2 protein. VEGFR2 is a receptor tyrosine kinase that plays a crucial role in angiogenesis and vascular development. The phosphorylated form of VEGFR2 is an important signaling molecule in these processes.

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Lab products found in correlation

β actin, by Abcam (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Total vegfr2, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by GenDEPOT (1 mentions) Lysis buffer, by Cell Signaling Technology (1 mentions) Pdgf bb, by Merck Group (1 mentions) Gapdh, by Abcam (1 mentions) Vegf a, by Merck Group (1 mentions) P pdgfrβ, by Cell Signaling Technology (1 mentions) P src tyr416, by Cell Signaling Technology (1 mentions) T src, by Cell Signaling Technology (1 mentions) P src tyr527, by Cell Signaling Technology (1 mentions) Total pdgfrβ, by Cell Signaling Technology (1 mentions) Ecl chemiluminescence kit, by Beyotime (1 mentions) Total cell protein extraction kit, by Keygen Biotech (1 mentions) Vegfa, by Abcam (1 mentions) Bovine serum albumin (bsa), by Keygen Biotech (1 mentions) Erk1 2, by Abcam (1 mentions) β actin, by Proteintech (1 mentions) Vegfr2, by Abcam (1 mentions)

Spelling variants of this product

VEGFR2 (57 citations) Anti-p-VEGFR2 (2 citations)

5 protocols using p vegfr2

Protein Expression and Immunoblotting

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Cells were lysed with lysis buffer (catalog no. #9803s; Cell Signaling Technology) containing protease inhibitor cocktail (catalog no. #3100-001; Gendepot). Total protein was immunoblotted with primary antibodies against KAI1 (catalog no. ab135779; Abcam), LIF (catalog no. AB-449-NA; R&D Systems), tSrc, pSrc (Tyr⁴¹⁶), and pSrc (Tyr⁵²⁷) (Cell Signaling Technology), Myc (catalog no. 05-724; Sigma-Aldrich), p53 (catalog no. MABE327; Merck Millipore), Pbx1 (catalog no. sc-889; Santa Cruz), Cav-1 (Caveolin-1, catalog no. sc-894; Santa Cruz), Flot1 (Flotillin1, catalog no. #3253s; Cell signaling Technology), p-VEGFR2 (catalog no. ab38473; Abcam), total VEGFR2 (catalog no. #2479s; Cell Signaling Technology), p-PDGFRβ (catalog no. #3166s; Cell signaling Technology), total PDGFRβ (catalog no. #4564s; Cell signaling Technology), PDGF-BB (catolg no. 07-1437; Sigma-Aldrich), and VEGF-A (catalog no. #ABS82; Merck Millipore) followed by incubation in horseradish peroxidase-conjugated secondary antibodies (Jackson Laboratory). GAPDH (catalog no. ab9485; Abcam) and beta-actin (catalog no. ab8227; Abcam) were used as an internal control.

Lee J.W., Hur J., Kwon Y.W., Chae C.W., Choi J.I., Hwang I., Yun J.Y., Kang J.A., Choi Y.E., Kim Y.H., Lee S.E., Lee C., Jo D.H., Seok H., Cho B.S., Baek S.H, & Kim H.S. (2021). KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state. Journal of Hematology & Oncology, 14, 148.

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Western Blotting of GSC Proteins

Cited 1 time

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Western blotting was performed as previously described [23 (link)]. Briefly, the total proteins of GSCs or tissues were isolated using a total cell protein extraction kit (KeyGen Biotechnology, Nanjing, China). Protein lysates were prepared, subjected to SDS-PAGE, transferred onto polyvinylidene difluoride membranes and blocked with 2% bovine serum albumin (KeyGen Biotechnology). The primary antibodies against ISL2 (1:1000; Abcam), VEGFA (1:1000; Abcam), VEGFR2 (1:1000; Abcam), p-VEGFR2 (1:1000; Abcam), MEK1/2 (1:1000; Abcam), p-MEK1/2 (1:1000; Abcam), ERK1/2 (1:500; Abcam), p-ERK1/2 (1:500; Abcam), and β-actin (1:2000; Proteintech, Rosemont, IL, USA) were incubated at 4 °C overnight. After secondary antibody (Proteintech) incubation, the bands were detected using a chemiluminescence ECL kit (Beyotime Biotechnology, Beijing, China) and quantified by ImageJ software (National Institutes of Health, Bethesda, MD, USA).

Jiang Y., Zhou J., Zhao J., Zhang H., Li L., Li H., Chen L., Hu J., Zheng W, & Jing Z. (2020). The U2AF2 /circRNA ARF1/miR-342–3p/ISL2 feedback loop regulates angiogenesis in glioma stem cells. Journal of Experimental & Clinical Cancer Research : CR, 39, 182.

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Multicolor Immunofluorescence Staining of Skin Tissue

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Skin tissue sections were incubated with two unconjugated primary antibodies, anti-CD31 (Cat#sc-1506, Santa Cruz Biotechnology, TX, USA) and anti-DA D₂ receptor antibody (Cat#sc-9113, Santa Cruz Biotechnology) or anti-CD31 (Cat# sc-1506, Santa Cruz Biotechnology) and anti-phospho-vascular endothelial growth factor receptor-2 (p-VEGFR-2) (Cat# ab5473, Abcam) or anti-CD31 (Cat# sc-1506, Santa Cruz Biotechnology) and anti-nidogen 2 (NID2) (Cat# ab14513, Abcam) or anti-CD31 (Cat# sc-1506, Santa Cruz Biotechnology) and anti-Apelin (Cat# 11497–1-AP, Life Technologies, CA, USA) at 4°C overnight. All the antibodies were used at a dilution of 1:100). The corresponding fluorochrome-conjugated secondary antibodies were applied for 1 hour at room temperature on the next day, and DAPI was used to counterstain the nuclei. Slides were mounted and visualized under a confocal scanning microscope (FBV-1000; Olympus Corporation, Center Valley, PA) (6 (link), 13 (link)).

Lu K., Bhat M., Peters S., Mitra R., Mo X., Oberyszyn T.M., Dasgupta P.S, & Basu S. (2021). Dopamine prevents ultraviolet B-induced development and progression of premalignant cutaneous lesions through its D2 receptors. Cancer prevention research (Philadelphia, Pa.), 14(7), 687-696.

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Anlotinib Hydrochloride Pharmacological Evaluation

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Anlotinib hydrochloride (purity >99%) was manufactured by Chia Tai Tianqing Pharmaceutical Co, Ltd (Nanjing, China) and dissolved in physiological saline to various concentrations. Primary and secondary antibodies against VEGFR2, p-VEGFR2, and β-actin were purchased from Abcam (Cambridge, MA, USA). β-catenin and c-Myc were provided by Cell Signaling Technology (Danvers, MA, USA). Matrigel was obtained from BD Bioscience (Pasadena, CA, USA). Tris-buffered saline with 0.1% Tween-20 (TBST) was purchased from Sigma Aldrich.

Gu G., Hu C., Hui K., Chen T., Zhang H, & Jiang X. (2021). NEAT 1 knockdown enhances the sensitivity of human non-small-cell lung cancer cells to anlotinib. Aging (Albany NY), 13(10), 13941-13953.

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Comprehensive Immunophenotyping and Signaling Analysis

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For IHC, antibodies used were from Cell Signaling Technology for CD31 (#77699S) and for CD8 (#98941S). For immunoprecipitation performed for Western blot analysis, antibodies used were from R&D Systems [Goat-anti-mouse antibody, VEGFR2 (#AF644) and AXL (#AF154)]. For Western blot analysis, antibodies used were from Cell Signaling Technology [VEGFR2 (#9698), pAXL (#5724), and cMET (#4560S)], Thermo Fisher Scientific [pVEGFR2 (#44-1047G) and pMET (#44-88G)] and Abcam [AXL (#ab215205)].
For flow cytometry, antibodies used for surface staining were: PD-L1 (MIH5; Thermo Fisher Scientific), PD-1 (29F.1A12; BioLegend), CD3 (145-2C11; BioLegend), CD11b (M1/70; BioLegend), CD45 (30-F11; BioLegend), CD8 (53-6.7; BioLegend), NKp46 (29A1.4; BioLegend), CD4 (RM4-5; BioLegend), Ly6G (1A8; BioLegend), CD11c (N418; Thermo Fisher Scientific), F4/80 (T45-2342; BD Biosciences), Ly6C (AL-21; BD Biosciences), MHC II (M5/114.15.2; BD Biosciences), CD19 (6D5; BioLegend), and CD25 (PC61; BioLegend). For intracellular staining, the antibodies used were: Arginase 1 (A1exF5; Thermo Fisher Scientific), Foxp3 (FJK-16s; eBioscience), Ki67 (Sola15; eBioscience), IFN (XMG1.2; BioLegend), and granzyme B (GB11; BioLegend).

Hsu J., Chong C., Serrill J., Goon L., Balayan J., Johnson E.N., Lorenzana G., Wu S., Leong K.G., Yun T.J., Wang Y., Jiang F., Bannen L., Lamb P., Xu W, & Yu P. (2022). Preclinical Characterization of XL092, a Novel Receptor Tyrosine Kinase Inhibitor of MET, VEGFR2, AXL, and MER. Molecular Cancer Therapeutics, 22(2), 179-191.

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