The levels of alanine aminotransferase (ALT) (ab234579, Abcam Inc., Cambridge, MA, USA), aspartate aminotransferase (AST) (ab263883, Abcam), IL‐6 (ab178013, Abcam), TNF‐α (ab181421, Abcam), IL‐1β (ab217608, Abcam) and IL‐18 (ab215539, Abcam) was detected using ELISA kits. Briefly, the sample to be tested (containing antibody) was bound to antigen, and then, the labelled enzyme was bound to the complex to form antigen‐antibody‐labelled enzyme complex. The enzyme substrate was added to produce coloured product, and its optical density value was determined by spectrophotometer.
Ab234579
Manufactured by Abcam
Sourced in United States
Ab234579 is a lab equipment product manufactured by Abcam. It is designed to perform a core function in laboratory procedures. Detailed information about its specific features and applications is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Ab263883, by Abcam (7 mentions)
Ab108789, by Abcam (3 mentions)
Mbs730053, by MyBioSource (2 mentions)
Ab178013, by Abcam (1 mentions)
Ab217608, by Abcam (1 mentions)
Ab215539, by Abcam (1 mentions)
Ab181421, by Abcam (1 mentions)
Total protein, by Bio-Rad (1 mentions)
Csb e04494r, by Cusabio (1 mentions)
Ab255730, by Abcam (1 mentions)
Ab236712, by Abcam (1 mentions)
Ab214566, by Abcam (1 mentions)
Ab234570, by Abcam (1 mentions)
Mak066, by Merck Group (1 mentions)
Mak085, by Merck Group (1 mentions)
S0024, by Beyotime (1 mentions)
Ab263882, by Abcam (1 mentions)
Ab34139, by Abcam (1 mentions)
Ab100747, by Abcam (1 mentions)
Ab100712, by Abcam (1 mentions)
8 protocols using ab234579
1
Quantifying Inflammatory Markers via ELISA
2
Serum Biomarker Quantification
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Serum was utilized to measure the levels of ALT, AST, and albumin by the ab234579, ab263883, and ab108789 kits purchased from Abcam, USA. In addition, the total proteins (BioRad, Hercules, CA, USA) and bilirubin (MBS730053, MyBioSource, San Diego, CA, USA) were measured in all experimental groups.
3
Serum-based Liver Function Assessment
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Serum was utilized, in the current study, for assessment of liver function using commercial kits of ELISA. The levels of alanine aminotransferase (ALT) (ab234579, Abcam, Cambridge, UK), aspartate aminotransferase (AST) (ab263883, Abcam, Cambridge, UK), albumin (ab108789, Abcam, Cambridge, UK), total proteins (BioRad, Hercules, CA, USA), and bilirubin (MBS730053, MyBioSource, San Diego, CA, USA) were measured in all groups [24 (link)].
4
Plasma Biomarkers Quantification Protocol
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Plasma levels of ACE (MBS2020292, MyBioSource, San Diego, CA, USA), AngII (CSB-E04494r, Cusabio, Houston, TX, USA), ALT (ab234579, Abcam, Waltham, MA, USA) and AST (ab263883, Abcam, Waltham, MA, USA) were measured via ELISA according to manufacturer’s instructions by a blinded researcher.
5
Liver Function and Inflammatory Markers
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Enzyme-linked immunosorbent assay (ELISA) kits of AST (ab263883, abcam, USA), ALT (ab234579, abcam, USA), tumor necrosis factor (TNF)-α (ab236712, abcam, USA), interleukin (IL)-1β (ab255730, abcam, USA), IL-6 (ab234570, abcam, USA) and IL-10 (ab214566, abcam, USA), and LDH kit (MAK066, Merck, German) were used to detect the levels of liver function markers and inflammatory factors in the serum according to the manufacturer’s instructions.
After washing the liver with a normal saline solution, 100 mg of liver tissue was cut and made into a 10% homogenate. The homogenate was centrifuged at 2,500 r/minute for 15 minutes to obtain the supernatant. The malondialdehyde (MDA) kit (MAK085, Merck, German), superoxide dismutase (SOD) kit (MM-0386R2, MEIMIAN, China), and nitric oxide (NO) kit (S0024, Beyotime, China) were used to detect the levels of MDA, SOD, and NO, respectively, in the supernatant.
After washing the liver with a normal saline solution, 100 mg of liver tissue was cut and made into a 10% homogenate. The homogenate was centrifuged at 2,500 r/minute for 15 minutes to obtain the supernatant. The malondialdehyde (MDA) kit (MAK085, Merck, German), superoxide dismutase (SOD) kit (MM-0386R2, MEIMIAN, China), and nitric oxide (NO) kit (S0024, Beyotime, China) were used to detect the levels of MDA, SOD, and NO, respectively, in the supernatant.
6
Serum Biomarker Profiling in Rats
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On week 4, blood samples (1 ml) were collected through cardiac puncture during sacrifice and serum was separated using centrifugation at 10,000 × g for 5 min at 4°C. Serum levels of TNF-α (ab100747, Abcam), IL-1β (ab100705, Abcam), IL-17A (ab199081, Abcam), IL-6 (ab100712, Abcam), TGF-β1 (ab118557, Abcam), IL-2 (ab223588, Abcam), IL-10 (ab33471, Abcam), ALT (ab234579, Abcam), AST (ab263882, Abcam), γ-GT (ab134640, Abcam), ALP (ab256583, Abcam), DBIL (ab34139, Abcam) and TBIL (ab37068, Abcam) were measured by using rat-specific sandwich ELISA (Abcam) according to the manufacturer's protocol.
7
Evaluating Hepatoprotective Effects of Mel-PLGA NPs
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Liver function parameters were measured in order to evaluate the hepatoprotective effect of Mel-PLGA NPs. The levels of aspartate aminotransferase (AST), alanine transaminase (ALT), albumin (ab234579, ab263883, ab108789, abcam, USA) and total bilirubin (MBS9389057, MyBiosource, USA) were measured by rat ELISA kits according to Farid et al.27 .
8
Quantifying Cardiac Biomarkers
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Corresponding enzyme-linked immunosorbent assay kits (ab246529, ab285275, ab263883, and ab234579; Abcam) were employed to measure the contents of Troponin I, creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). The aforementioned biochemical indexes were measured strictly per the specific operation steps and cautions in the protocols of the corresponding kits. The optical density was analyzed and calculated with a microplate reader (Beckman Coulter, CX7, Brea, CA, USA) and the concentrations of the samples were calculated based on the corresponding formula or standard curve and regression equation of biomarkers.
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