Sterile titanium screws measuring 1/16 inch (Antrin Miniature Specialties Inc., Fallbrook, CA) were charge modified using the LbL assembly method. A total of six polyelectrolyte bilayers were assembled. To confirm charge, negatively charged Alexafluor® 350 hydrazide sodium salt dissolved in 200mM NaCl to a final concentration of 1mM (Molecular Proves, Eugene, OR, USA) was bound to unmodified, positively modified and negatively modified screws followed by rinsing with water. The screws were imaged on a Leica optical microscope (DM4000M) (Leica Camera, Wetzlar, Germany) using a filter with excitation range 340-380 nm and emission wavelength of 425 nm.
Optical microscope dm4000m
Manufactured by Leica
Sourced in Germany
The Leica DM4000M optical microscope is a high-performance instrument designed for advanced microscopy applications. It features a stable and ergonomic design, providing a reliable platform for detailed observation and analysis. The microscope is equipped with a range of optical systems, including bright-field, dark-field, and phase contrast modes, enabling the user to select the appropriate technique for their specific needs.
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Lab products found in correlation
2 protocols using optical microscope dm4000m
1
Charge Modification of Titanium Screws
2
Paper Substrate Extinction Spectra
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Extinction spectra from paper substrates were collected using a CRAIC microspectrophotometer (QDI 302) coupled to a Leica optical microscope (DM 4000 M) with 20× objective in the range of 450–800 nm with 10 accumulations and 100 ms exposure time in reflection mode. The spectral resolution of the microspectrophotometer is 0.2 nm. Multiple UV-Visible spectra (~10) were collected from different locations of the paper strip before and after exposure to troponin solution. Shimadzu UV-1800 spectrophotometer was employed to collect UV-Vis extinction spectra from solution.
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