Zorbax 300 sb c18 5 micron

Anonymous human EDTA plasma with no identifying information were received and analyzed under the Ryerson Ethical Review Board Protocol REB 2015-207: ICU-Sepsis versus ICU Control EDTA plasma were obtained from Clinical Evaluation Research Unit, Kingston General Hospital, Kingston Ontario Canada; Ovarian and breast cancer samples were obtained from the Ontario Tumor bank of the Ontario Institute of Cancer Research, Toronto Ontario; Heart attack (venous and arterial) versus pre-operative orthopedic surgery controls were obtained from St Joseph’s Hospital of McMaster University; Multiple sclerosis Alzheimer’s dementia and institution-matched normals were obtained from Amsterdam University Medical Centers, Vrije Universiteit Amsterdam; In addition, EDTA plasma samples collected onto ice as a baseline degradation controls were obtained from IBBL Luxembourg [39 (link), 40 (link)]. C18 zip tips were obtained from Millipore (Bedford, MA), C18 HPLC resin was from Agilent (Zorbax 300 SB-C18 5-micron). Solvents were obtained from Caledon Laboratories (Georgetown, Ontario, Canada). All other salts and reagents were obtained from Sigma-Aldrich-Fluka (St Louis, MO) except where indicated. The level of replication in the LC–ESI–MS–MS experiments was typically between 9 to 26 independent patient plasma samples (Additional file 1: Table S1).

Human EDTA plasma with no identifying information were received and analyzed under the Ryerson Ethical Review Board Protocol REB 2015-207: Treatment-blinded, Alzheimer’s dementia (AD), Multiple sclerosis (MS) and institution-matched normals were obtained from Amsterdam University Medical Centers, Vrije Universiteit Amsterdam; ICU-Sepsis versus ICU Control EDTA plasma were obtained from Clinical Evaluation Research Unit, Kingston General Hospital, Kingston Ontario Canada; Ovarian and breast cancer samples along with female only controls were obtained from the Ontario Tumor bank of the Ontario Institute of Cancer Research, Toronto Ontario; Heart attack (venous and arterial) versus pre-operative orthopedic surgery controls were obtained from St Joseph’s Hospital of McMaster University; In addition, EDTA plasma samples collected onto ice as a baseline degradation controls were obtained from IBBL Luxembourg [49 (link), 65 (link)]. C18 zip tips were obtained from Millipore (Bedford, MA), C18 HPLC resin was from Agilent (Zorbax 300 SB-C18 5-micron). Solvents were obtained from Caledon Laboratories (Georgetown, Ontario, Canada). All other salts and reagents were obtained from Sigma-Aldrich-Fluka (St Louis, MO) except where indicated.

The 1100 HPLC system was from Agilent (Santa Clara, CA, USA). The LTQ XL linear ion traps were obtained from Thermo Electron Corporation (Waltham, MA, USA). The random spectra generator was slightly modified from that provided by Zhu et al. [28 (link)]. The EDTA blood sample tubes were from Becton Dickinson (B367844 K2EDTA, 7.2 mg) (Franklin Lakes, NJ, USA). The C18 with 5 micron particle size and 300 Angstrom pore size was from Agilent Zorbax 300 SB-C18 5-micron (Agilent). The HPLC grade water and acetonitrile was from Caledon laboratories and the formic acid from Fluka (Georgetown, ON, Canada). Sequencing grade trypsin was from Roche (Basel, Switzerland). The ZipTip C18 micro preparative column (Millipore C18 ZipTips, cat # ZTC 18S 096, peptide capacity 5 µg) were from Millipore (Billerica, MA, USA).