Tetraethyl orthosilicate (TEOS, 99 wt.%), cetyltrimethylammonium bromide (CTAB), N,N’-dicyclohexylcarbodimide (DCC, >98%), 2-N-morpholino-ethanesulfonic acid (MES), 4-dimethylaminopyridine (DMAP) and N-hydroxysuccinimide (NHS, >98%) were purchased from Aladdin. 2-cyanoethyltriethoxysilane (CETES), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-HCl (EDC, >98%), epsilon-aminocaproic acid (ACA), 2-hydroxyethyl trimethylammonium chloride (ChCl), Fluorescein isothiocyanate (Flu) and acetylcholine esterase (AChE) were purchased from Sigma-Aldrich. p-Sulfonatocalix[6]arene (p-SC6A) was obtained from TCI Development Co., Ltd. (Shanghai, China). 1,2,3,4-Tetrahydro-5-aminoacridine hydrochloride (Tacrine, Tac) was purchased from Heowns Biochem. All these reagents were used as received without further purification. Deionized water (18.2 MΩ cm) used for all experiments was obtained from a Milli-Q system (Millipore, Bedford, MA).
C57BL6/J mice were obtained from the Experimental Animal Center of the Chinese Academy of Medical Sciences. Bel7402 cells were obtained from American Type Culture Collection. RPMI 1640 and fetal bovine serum (FBS) were purchased from Gibco BRL. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was bought from Sigma-Aldrich.
C57BL6/J mice were obtained from the Experimental Animal Center of the Chinese Academy of Medical Sciences. Bel7402 cells were obtained from American Type Culture Collection. RPMI 1640 and fetal bovine serum (FBS) were purchased from Gibco BRL. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was bought from Sigma-Aldrich.