The RNAi kits of HAT1 and Fas, antibodies of Fas and HAT1 were purchased from Santa Cruz Biotech (Santa Cruz, CA). The recombinant TNF, FasL, PAR2-activating peptides SLIGRL and control peptide LRGILS were purchased from R&D Systems (Minneapolis, MN). The Annexin V kit and propidium iodide were purchased from Sigma Aldrich (St. Louis., MO). The reagents for performing RT-qPCR, Western blotting and gene transfection were purchased from Invitrogen (Carlsbad, CA). Immune cell isolation reagent kits were purchased from Miltenyi Biotech (San Diego, CA).
Annexin 5 kit
Manufactured by Merck Group
Sourced in United States
The Annexin V kit is a laboratory reagent used to detect and quantify apoptosis, a form of programmed cell death. It utilizes the calcium-dependent binding of the Annexin V protein to phosphatidylserine, which becomes exposed on the cell surface during apoptosis. The kit provides the necessary components to perform this assay.
Automatically generated - may contain errors
Lab products found in correlation
Chip kit, by Merck Group (3 mentions)
Propidium iodide, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Immune cell isolation reagent kits, by Miltenyi Biotec (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Sodium butyrate, by Merck Group (1 mentions)
Phdac1, by Santa Cruz Biotechnology (1 mentions)
Hdac1, by Santa Cruz Biotechnology (1 mentions)
Fvd efluor780, by Thermo Fisher Scientific (1 mentions)
Binding buffer 1x, by Merck Group (1 mentions)
Pol 2, by Santa Cruz Biotechnology (1 mentions)
Protein g agarose, by Merck Group (1 mentions)
Bcl2l12, by Santa Cruz Biotechnology (1 mentions)
Tgf β, by R&D Systems (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti p erk, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti p53, by Cell Signaling Technology (1 mentions)
15 protocols using annexin 5 kit
1
Examining Immune Cell Responses Using RNAi and Antibody Techniques
2
Apoptosis Analysis of Gemcitabine and Entinostat
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7.5×105 cells were seeded in 24-well plates and allowed to adhere overnight before adding indicated amounts of gemcitabine and/or entinostat. After 72 hours cells were harvested and stained for annexin V (early apoptosis marker) and propidium iodide (PI) (late apoptosis) using the Annexin V kit (Sigma-Aldrich) according to the manufacturer's protocol followed by flow cytometric analysis.
3
Evaluating SOD1 Inhibitor and Modulators
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SOD1 inhibitor (ATN224, 15 μM for cell culture; 4 mg/kg i.p. for mouse model study) was purchased from Baiolibo Inc. (Beijing, China). p38 inhibitor SB203580 was obtained from Calbiochem (San Diego, CA). Indicaxanthin was purchased from Molbase Biotech (Shanghai, China). Antibodies (Abs) of SOD1 (SOD, in short; clone# B-1), CD5 (UCH-T2, AF546), FasL (Kay-10), CD20 (D-10), STAT3 (F-2), pSTAT3 (B-7), CD19 (B-1, AF488), and IL-10 (3C12C12, AF647) were purchased from Santa Cruz Biotech (Santa Cruz, CA). Abs of pJNK (T183), NF-κB (E39), pNF-κB (EP2294Y), p38 (9F12), and p-p38 (EPR269Y) were purchased from Abcam (Cambridge, MA). ELISA kits of ROS, SOD, IL-4, IL-5, IL-13, IL-10, tryptase, mMCP1, ECP, and OVA-specific IgE were purchased from Biocompare (South San Francisco, CA). GL7 Ab (APC) was purchased from Dakewe BioMart (Beijing, China). Annexin V kit, OVA, and FGN were purchased from Sigma-Aldrich (St. Louis, MO). Extracts of food allergens (cow's milk, egg white, egg yolk, wheat flour, soybean, carrot, potato, and peanut) were purchased from Allergopharma (Germany).
4
Molecular Techniques in Cancer Research
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The antibodies of Trib1, HDAC1, pHDAC1, p53, and shRNA kits of Trib1 and HDAC1 were from Santa Cruz Biotech (Shanghai, China). The Annexin V kit, protein G, butyrate sodium and Chip kit were from Sigma Aldrich (Shanghai, China). The reagents for RT-qPCR and Western blotting were from Invitrogen (Shanghai, China).
5
Exploring Apoptosis-Related Regulatory Pathways
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The Abs against survivin, Fas, FasL, MBP, IL-4 receptor α (IL-4Rα), c-Myc, and
IL-4α RNAi kit were purchased from Santa Cruz Biotech (Santa Cruz, CA). The
YM-155, survivin, IL-4, IL-5, IL-13, and survivin ELISA kit were purchased from
BioMart (Beijing, China). The annexin v kit, propidium iodide (PI),
FITC-dextran, reagents, and materials for immunoprecipitation (IP) and chromatin
IP (ChIP) were purchased from Sigma Aldrich (St. Louis, MO). The reagents and
materials for RT-qPCR and Western blotting were purchased from Invitrogen
(Carlsbad, CA).
IL-4α RNAi kit were purchased from Santa Cruz Biotech (Santa Cruz, CA). The
YM-155, survivin, IL-4, IL-5, IL-13, and survivin ELISA kit were purchased from
BioMart (Beijing, China). The annexin v kit, propidium iodide (PI),
FITC-dextran, reagents, and materials for immunoprecipitation (IP) and chromatin
IP (ChIP) were purchased from Sigma Aldrich (St. Louis, MO). The reagents and
materials for RT-qPCR and Western blotting were purchased from Invitrogen
(Carlsbad, CA).
6
Apoptosis Assay of Calu-6 Cells
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Calu-6 cells infected or not with the strains (MOI of 5) and PMN treated or not with CM and then infected with H37Rv (MOI of 3) were cultured for further 18 h, detached from the 96-well tissue culture plates, and washed to obtain the pellet. Then Calu-6 and PMN cellular pellets were stained with a fixable viability dye FVD eFluor 780 (eBioscience) and vortexed immediately. After 30 min of incubation at −4°C and protected from light, cells were washed with Binding Buffer 1X (Sigma) and stained with Annexin V kit (Sigma Aldrich) according to the manufacturer instructions. After the time of incubation with Annexin V, cells were washed and fixed with PFA 1% for 20 min and washed with Binding Buffer 1X (Sigma Aldrich). Finally, cells were suspended in flow cytometry buffer and acquired at the cytometer. Results are expressed as percentage of positive or negative cells for Annexin V and FVD eFluor 780.
7
Studying Livin and Apoptosis Signaling
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RNAi kit of livin, antibodies of livin, Fas, HSF1, Pol II and ubiquitin were purchased from Santa Cruz Biotech (Santa Cruz, CA). FITC labeled anti-Fas antibody was purchased from BD Biosciences (Franklin Lakes, NJ). ChIP kit, materials for immunoprecipitation and Annexin V kit was purchased from Sigma Aldrich (St. Louis., MO). Reagents and materials for RT-qPCR and Western blotting were purchased from Invitrogen (Carlsbad, CA).
8
Analyzing Cell Signaling Pathways
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The antibodies of Bcl2L12, p53 and Mdm2 were purchased from Santa Cruz Biotech (Santa Cruz, CA). The Annexin V kit, protein G agarose and ChIP kit were purchased from Sigma Aldrich (St. Louis., MO). The reagents for RT-qPCR and Western blotting were purchased from Invitrogen (Carlsbad, CA). The PAR2 active peptide (AP) and control peptide (CP) were provided by Enke Biotech (Shenzhen, China).
9
Multiplex Immune Response Analysis
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The fluorochrome-labeled antibodies of CD4, Foxp3 and IL-9 were purchased from BD Biosciences (Shanghai, China). The ELISA kits of IL-4, IL-5, IL-13, IFN-γ, IgE, terminal deoxynucleotidyltransferased dUTP nick end labeling (TUNEL) kit, neutralizing anti-IL-4 and anti-TGF-β were purchased from R&D Systems (Shanghai, China). The Annexin V kit was purchased from Sigma Aldrich (Shanghai, China). The immune cell isolation kits were purchased from Myltenyi Biotech (Guangzhou, China). The anti-Ly5.2 antibody was purchased from Santa Cruz Biotech (Santa Cruz, CA).
10
Apoptosis Assay for Cancer Cells
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The chemicals and reagents were not purified by further procedures before being used. Delphinidin chloride, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and anti-LC3 (#L7543) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-caspase 3 (#9662), anti-cleaved caspase 3 (#9664), anti-PI3K (#4249), anti-p-PI3K (#4228), anti-AKT (#4691), anti- p-AKT (#4060), anti-mTOR (#2983), anti-p-mTOR (#5536), anti-ERK (#4695), anti-p-ERK (#4377), anti-p38 (#9212), anti-p-p38 (#9215), anti-JNK (#9252), anti-p-JNK (#9251), anti-p53 (#9282), and anti-GAPDH (#2118) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The antibody for DRAM (ab188648) was purchased from Abcam (Cambridge, UK). RPMI 1640, penicillin/streptomycin (P/S), and foetal bovine serum (FBS) were purchased from Lonza (Walkersville, MD, USA). The Annexin V kit was purchased from Millipore (San Francisco, CA, USA).
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