Aa 6880f

For the determination of some minerals (Ca, Mg, Fe, Zn, and Cu), a 1.0 g anchote tuber flour was calcinated in a muffle furnace (Nabertherm, D‐6072 Dreieich, Germany) at 450°C for 4 hr. The ash was dissolved in 20% nitric acid and filtered using the Whatman filter paper (42 mm) before analysis. Then, the minerals Ca, Mg, Fe, Zn, and Cu were determined using atomic absorption spectrophotometer (SHIMADZU, AA‐6880F, Japan) using air/acetylene flame at wavelengths of 422.7, 285.2, 248.3, 213.9, and 324.7 nm respectively. Finally, the concentrations of the elements in the samples were calculated using the following equation.
$\text{Concentration}\left(\text{mg}/100g\right)=\frac{\left(a‐b\right)xV}{10xWs}$ where a = concentration in ppm (mg/L) of sample; b = concentration in ppm (mg/L) of blank solution, V = Volume in ml of extract, Ws = Weight of sample in g, 10 = conversion factor.

Minerals such as iron, calcium, and zinc were analyzed using Atomic Absorption Spectrophotometer (AAS) (SHIMADZU, AA-6880F, Japan) following Jorhem (2000) (link) method. Zinc, iron, and calcium are major limiting nutrients that are currently regarded as major nutrients and public health concerns in Ethiopia (EPHI, 2016 , Umeta et al., 2005 ).