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Bc0595

Manufactured by Solarbio
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Sourced in China

BC0595 is a laboratory equipment product. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Bc0625, by Solarbio (4 mentions) Bc0345, by Solarbio (4 mentions) Bc0685, by Solarbio (4 mentions) Bc0955, by Solarbio (4 mentions) Bc0305, by Solarbio (2 mentions) Bc0730, by Solarbio (1 mentions) Bc0980, by Solarbio (1 mentions) Catalyst dx, by IDEXX (1 mentions) Biotek synergy h1, by Agilent Technologies (1 mentions) Bc0590, by Solarbio (1 mentions)

11 protocols using bc0595

1

Skeletal Muscle Metabolite and Enzyme Analysis

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Content of adenosine triphosphate (ATP), triglyceride (TG), free fatty acid (FFA), glycogen, and acetyl-CoA as well as enzyme activity of lactic dehydrogenase (LDH), succinate dehydrogenase (SDH), acetyl-CoA carboxylase (ACC) and pyruvate carboxylase (PC) in skeletal muscle were measured using commercially available kits (BC0305, BC0625, BC0595, BC0345, BC0980, BC0685, BC0955, BC0410 and BC0730, Solarbio, China) according to the manufacturer’s instructions.
Cai B., Ma M., Zhang J., Kong S., Zhou Z., Li Z., Abdalla B.A., Xu H., Zhang X., Lawal R.A, & Nie Q. (2022). Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development. Cell Death & Disease, 13(4), 389.
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2

Skeletal Muscle Metabolic Analysis

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Content of TG, FFA, and glycogen as well as enzyme activity of LDH and SDH in skeletal muscle were measured using commercially available kits (BC0625, BC0595, BC0345, BC0685, BC0955, respectively; Solarbio, China), according to the manufacturer’s instructions.
Ma M., Cai B., Zhou Z., Kong S., Zhang J., Xu H., Zhang X, & Nie Q. (2023). LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers. Cell Communication and Signaling : CCS, 21, 7.
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3

NEFA Quantification Assay Protocol

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Non-esterified free fatty acids (NEFA) were measured using a commercially available assay according to the manufacturer s instructions (BC0595, Beijing Solarbio). A standard curve was made from 0.05 to 1 μmol/mL serial dilution in a 96-well plate, then followed by adding 10 μL of each group sample, finally addition of the manufacturer s reagents, and the absorbance value was measured at 550 nm.
Gu Y., Niu X., Yin L., Wang Y., Yang Y., Yang X., Zhang Q, & Ji H. (2021). Enhancing Fatty Acid Catabolism of Macrophages Within Aberrant Breast Cancer Tumor Microenvironment Can Re-establish Antitumor Function. Frontiers in Cell and Developmental Biology, 9, 665869.
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4

Skeletal Muscle Metabolite Analysis

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Content of TG, FFA, ATP, and glycogen as well as enzyme activity of LDH and SDH in skeletal muscle were measured using commercially available kits (BC0625, BC0595, BC0305, BC0345, BC0685, BC0955, respectively; Solarbio, China), according to the manufacturer's instructions.
Cai B., Ma M., Zhang J., Wang Z., Kong S., Zhou Z., Lian L., Zhang J., Li J., Wang Y., Li H., Zhang X, & Nie Q. (2021). LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with SERCA2. Molecular Therapy. Nucleic Acids, 27, 319-334.
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5

Quantification of Free Fatty Acids in Breast Cancer Cells

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The content of free fatty acid (FFA) in breast cancer cells was measured using commercially available kits (BC0595, Solarbio, China) according to the manufacturer's instructions. According to the protocol, cells (5 × 106) were broken in 1 mL extracting solution by sonicator and centrifugated at 8000 rpm, 4 °C for 10 min. The upper phase was collected and placed in ice for the next step. A reaction system containing 30 μl samples, 300 μl reagent 1 (n-heptane: anhydrous methanol: chloroform = 24:1:25), and 120 μl reagent 2 (37 °C) oscillated for 10 min to centrifugate at 3000 rpm. A 50 μl upper liquid and 200 μl reagent 3 were mixed to oscillate for 2 min, and stand for 15 min. Finally, 200 μl samples were determined in 96-well plates to obtain the absorbance at 550 nm.
Xia Z., Zhao N., Liu M., Jiang D., Gao S., Ma P, & Huang L. (2023). GPD1 inhibits the carcinogenesis of breast cancer through increasing PI3K/AKT-mediated lipid metabolism signaling pathway. Heliyon, 9(7), e18128.
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6

Circulating Lipid Biomarker Analysis

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An automatic chemical chemistry analyzer (Catalyst Dx, IDEXX, USA) was used to measure concentrations of circulating triglycerides (98-11086-01, Catalyst, USA). Circulating glycerol (XFP17067, GEMIC, Nanjing, China) and circulating free fatty acids (#BC0595, Solarbio, Beijing, China) were determined according to the manufacturer’s instructions.
Wang T., Zhao C., Zhang J., Li S., Zhang Y., Gong Y., Zhou Y., Yan L., Zhang S., Zhang Z., Hu H., Liu A., Bai X, & Zou Z. (2024). Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9. iScience, 27(2), 108857.
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7

Lipid Profiling Protocol for Biomedical Research

Cited 1 time
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Plasma triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured following the methods and assay kits of our previous study [32 (link)]. Plasma non-esterified fatty acids (NEFA) were determined using a commercially available kit from Solarbio (BC0595, Beijing, China). Changes in absorbance were determined with Bio Tek Synergy H1 (Bio Tek Instruments, Inc., Winooski, VT, USA) at 550 nm.
Wang Y., Lavier J., Hua W., Gong L., Wei H., Wang J., Pellegrin M., Millet G.P, & Zhang Y. (2022). Effects of Six Weeks of Hypoxia Exposure on Hepatic Fatty Acid Metabolism in ApoE Knockout Mice Fed a High-Fat Diet. Life, 12(10), 1535.
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8

Quantifying FFA and Insulin Levels

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The concentrations of FFA (Solarbio, Cat# BC0595) were determined using the corresponding kits as indicated in their instructions. Insulin was determined by ELISA (CrystalChem, Cat# 90080), according to the manufacturer's instructions.
Zhou W.Y., Liu P., Xia Y.F., Shi Y.J., Xu H.Y., Ding M., Yang Q.Q., Qian S.W., Tang Y., Lu Y., Tang Q.Q, & Liu Y. (2024). NR2F6 is essential for brown adipocyte differentiation and systemic metabolic homeostasis. Molecular Metabolism, 81, 101891.
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9

Serum Free Fatty Acid Assay

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The serum FFA levels were determined with a serum FFA measurement kit (BC0595, Solarbio, CN) following manufacturer’s instructions. A standard curve was established along with actual samples. After data acquisition with a plate reader (M5, MD-SpectraMax, Molecular Devices, San Jose, CA, USA) at 550 nm absorption, the serum concentration of FFA was calculated according to the standard curve.
Yuan J., Jiang Q., Song L., Liu Y., Li M., Lin Q., Li Y., Su K., Ma Z., Wang Y., Liu D, & Dong J. (2020). L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction. Molecules, 25(1), 176.
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10

Gastrocnemius Biomarker Quantification

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Content of free fatty acid (FFA), glycogen and triglyceride (TG), as well as enzyme activity of lactic dehydrogenase (LDH) and succinate dehydrogenase (SDH) in gastrocnemius were measured using commercially available kits (BC0595, BC0345, BC0625, BC0685, and BC0955, Solarbio, Beijing, China) following the manufacturer’s instructions.
Ma M., Cai B., Kong S., Zhou Z., Zhang J., Zhang X, & Nie Q. (2022). PPARGC1A Is a Moderator of Skeletal Muscle Development Regulated by miR-193b-3p. International Journal of Molecular Sciences, 23(17), 9575.
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