Performance high sensitivity human cytokine

Manufactured by R&D Systems

Sourced in United States

The Performance High Sensitivity Human Cytokine is a laboratory equipment designed for the detection and quantification of various cytokines in human samples. It utilizes a highly sensitive immunoassay technology to provide accurate and reliable measurements.

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Lab products found in correlation

Bio plex 200, by DiaSorin (3 mentions) Human crp quantikine elisa, by R&D Systems (1 mentions) Luminex, by R&D Systems (1 mentions)

7 protocols using performance high sensitivity human cytokine

Plasma Cytokine Quantification Protocol

Cited 3 times

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Whole blood samples were collected in pre-chilled EDTA tubes. After collection, the samples were centrifuged at 4°C, plasma was harvested into multiple aliquots, and then stored in a −70°C freezer until the completion of the study.
Plasma TNF-α and IL-6 concentrations (assay ranges 0.8–3100 pg/mL and 0.2–3800 pg/mL, respectively) were determined using a Bio-Plex 200 (Luminex) Instrument, and a high sensitivity bead-based multiplex immunoassay (Performance High Sensitivity Human Cytokine, R& D Systems, Minneapolis, MN), as previously described (Moieni et al., 2015a (link); Moieni et al., 2015b (link)). All plasma samples from each subject (baseline and all subsequent time points) were assayed on the same 96-well plate; every subject demonstrated the expected profile of change of cytokine concentrations over time, based on previous studies (Eisenberger et al., 2010 (link); Eisenberger et al., 2009 (link)). The mean intra-assay CV% of the standards was < 8% for TNF-α and IL-6; the inter-assay CV% of an internal laboratory quality control sample was < 13% for both analytes.

Moieni M., Irwin M.R., Jevtic I., Breen E.C., Cho H.J., Arevalo J.M., Ma J., Cole S.W, & Eisenberger N.I. (2015). Trait sensitivity to social disconnection enhances pro-inflammatory responses to a randomized controlled trial of endotoxin. Psychoneuroendocrinology, 62, 336-342.

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Plasma Cytokine Profiling Methodology

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Plasma tumor necrosis factor (TNF)-α and interleukin (IL)-6 concentrations were determined using a high sensitivity bead-based multiplex immunoassay (Performance High Sensitivity Human Cytokine, R&D Systems, Minneapolis, MN, United States), as previously described (Moieni et al., 2015a (link),b (link),c (link)). The full cytokine profile for participants from this exact study is available in other publications (Moieni et al., 2015b (link),c (link)).

Moieni M., Muscatell K.A., Jevtic I., Breen E.C., Irwin M.R, & Eisenberger N.I. (2019). Sex Differences in the Effect of Inflammation on Subjective Social Status: A Randomized Controlled Trial of Endotoxin in Healthy Young Adults. Frontiers in Psychology, 10, 2167.

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Multiplex Cytokine Profiling in Inflammation

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Peripheral inflammation was assessed at each time point as previously described [24 ]. In brief, plasma concentrations of interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α were measured using a Bio-Plex 200 (Luminex Corporation, Austin, TX) instrument and a high-sensitivity multiplex immunoassay (Performance High Sensitivity Human Cytokine, R&D Systems, Minneapolis, MN). Data acquisition and analyses were performed with Bio-Plex software v4.1, and a 5-parameter logistic curve fit. Multiplex assays, which are shown to have high intra-assay reproducibility [28 ], were performed on samples diluted twofold as per the manufacturer’s protocol. C-reactive protein (CRP) was measured using Human CRP Quantikine ELISA (R&D Systems), also as per manufacturer protocols.

Andreou B., Reid B., Lyall A.E., Cetin-Karayumak S., Kubicki A., Espinoza R., Kruse J., Narr K.L, & Kubicki M. (2022). Longitudinal trajectory of response to electroconvulsive therapy associated with transient immune response & white matter alteration post-stimulation. Translational Psychiatry, 12, 191.

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Quantification of IL-6 and TNF-α

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Plasma levels of IL-6 and TNF-α were quantified by high sensitivity bead-based multiplex (Luminex) immunoassays (Performance High Sensitivity Human Cytokine, R&D Systems, Minneapolis, MN, USA), as previously described.^{21 (link)}

Cho H.J., Eisenberger N.I., Olmstead R., Breen E.C, & Irwin M.R. (2016). Preexisting mild sleep disturbance as a vulnerability factor for inflammation-induced depressed mood: a human experimental study. Translational Psychiatry, 6(3), e750-.

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Cytokine Quantification in Plasma

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Plasma levels of interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) were quantified by high sensitivity bead-based multiplex (Luminex) immunoassays (Performance High Sensitivity Human Cytokine, R&D Systems, Minneapolis, MN, USA), as previously described (Moieni et al., 2015a (link); Moieni et al., 2015b (link)). The lower limits of detection for IL-6 and TNF-α were 0.2 and 0.8 pg/ml, respectively. TNF-α was detectable in 100% of samples. For the small proportion of samples with IL-6 below the lower limit of detection (<1%), the values were treated as missing. The mean intra-assay CV% of the standards was <8% for IL-6 and TNF-a; the interassay CV% of an internal laboratory quality control sample was <3% for both analytes. In the current study, analyses focused only T0, T2, and T6 time points, given that these are the time points for which KYN pathway data were available for comparison.

Kruse J.L., Cho J.H., Olmstead R., Hwang L., Faull K., Eisenberger N.I, & Irwin M.R. (2019). Kynurenine Metabolism and Inflammation-Induced Depressed Mood: A Human Experimental Study. Psychoneuroendocrinology, 109, 104371.

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Cytokine Profiling from Frozen Plasma

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Whole blood samples were collected in prechilled EDTA tubes. After collection, the samples were centrifuged at 4 °C, plasma was harvested into multiple aliquots, and then stored at − 70 °C until immunoassays were performed.
Data for baseline (T0) levels of IL-8, IL-6, and TNF-α were obtained by high sensitivity bead-based multiplex (Luminex) immunoassays (Performance High Sensitivity Human Cytokine, R&D Systems, Minneapolis, MN, USA), as previously described^{15 (link)}. Due to the strength of the original study design, which utilized up to seven repeated measures of cytokine values to profile inflammatory cytokine responses for each subject, the plasma sample from each time point was evaluated in a single determination. The average intra-assay CV% for standards was 4.3, 4.4, and 5.3 for IL-6, IL-8, and TNF-α, respectively; the inter-assay CV% of an internal laboratory quality control sample was < 13.5% for all three analytes. The lower limit of detection for IL-8 was 0.1 and values lower than this limit were entered as 0.05 (1/2 the lower limit); n = 6 samples).

Kruse J.L., Boyle C.C., Olmstead R., Breen E.C., Tye S.J., Eisenberger N.I, & Irwin M.R. (2022). Interleukin-8 and depressive responses to an inflammatory challenge: secondary analysis of a randomized controlled trial. Scientific Reports, 12, 12627.

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Plasma Cytokine Profiling in Clinical Study

Cited 4 times

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Whole blood samples were collected in pre-chilled EDTA tubes. After collection, the samples were centrifuged at 4°C, plasma was harvested into multiple aliquots, and then stored in a −70°C freezer until the completion of the study.
Plasma tumor necrosis factor (TNF)-α and interleukin (IL)-6 concentrations (assay ranges 0.8–3100 pg/mL and 0.2–3800 pg/mL, respectively) were determined using a Bio-Plex 200 (Luminex) Instrument, and a high sensitivity bead-based multiplex immunoassay (Performance High Sensitivity Human Cytokine, R& D Systems, Minneapolis, MN), as previously described (4 (link), 27 (link), 31 (link)). The full cytokine profile for participants from this exact study are available in other publications (4 (link), 27 (link)). All plasma samples from each subject (baseline and all subsequent time points) were assayed on the same 96-well plate; every subject demonstrated the expected profile of cytokine concentrations over time, based on previous studies (7 (link), 35 (link)). The mean intra-assay CV% of the standards was < 8% for TNF-α and IL-6; the inter-assay CV% of an internal laboratory quality control sample was < 13% for both analytes.

Moieni M., Tan K.M., Inagaki T.K., Muscatell K.A., Dutcher J.M., Jevtic I., Breen E.C., Irwin M.R, & Eisenberger N.I. (2019). Sex differences in the relationship between inflammation and reward sensitivity: A randomized controlled trial of endotoxin. Biological psychiatry. Cognitive neuroscience and neuroimaging, 4(7), 619-626.

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