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Anti batf d7c5

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Anti-BATF (D7C5) is a primary antibody that recognizes the BATF (Basic Leucine Zipper ATF-Like Transcription Factor) protein. BATF is a transcription factor that plays a role in regulating gene expression.

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Lab products found in correlation

Odyssey fc analyzer, by LI COR (2 mentions) Anti β actin 8h10d10, by Cell Signaling Technology (2 mentions) Criterion xt bis tris protein gel, by Bio-Rad (2 mentions) Anti mcpip1 antibody 604421, by R&D Systems (2 mentions) Anti ptpn2 e 11, by Santa Cruz Biotechnology (2 mentions) Anti socs1 e 9, by Santa Cruz Biotechnology (2 mentions) Anti hsp90 mab3286, by R&D Systems (2 mentions) Hrp conjugated anti mouse igg w4021, by Promega (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions) Anti gapdh 6c5, by Merck Group (1 mentions) Anti tubulin t9026, by Merck Group (1 mentions) Anti sox2, by R&D Systems (1 mentions) Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-BATF (5 citations) Anti-mouse BATF (D7C5) (2 citations)

3 protocols using anti batf d7c5

1

Analyzing Cell Proliferation and Protein Expression

Cited 1 time
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Whole-cell extracts were prepared in RIPA lysis buffer (13 (link)). Primary antibodies were as follows: anti-AID (16 (link)), anti-Sox2 (R&D Systems), anti-Gapdh (6C5; Millipore), anti-tubulin (T9026; Sigma), anti-Batf (D7C5; Cell Signaling Technology), and anti-p21 (B-2), anti-cyclinD2 (M-20), anti-Irf4 (M-17), all from Santa Cruz Biotechnology. Uninfected and retrovirally infected cells were stained with the CellTrace CFSE Cell Proliferation Kit (Molecular Probes) immediately following the first spin-infection (or at 24h of stimulation for control cells) according to the manufacturer’s instructions. Staining was detected by flow cytometry at 0h and 48h.
DiMenna L.J., Yen W.F., Nicolas L., Sharma R., Saldanha Z.N, & Chaudhuri J. (2017). The transcription factor Sox2 regulates AID expression in class switched B cells. Journal of immunology (Baltimore, Md. : 1950), 198(6), 2244-2248.
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2

Western Blot Profiling of Immune Regulators

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Cells were lysed in RIPA buffer (ThermoFisher 89900), resolved in 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad 3450124) and transferred to PVDF membrane (Bio-Rad 1620177). Membranes were blocked using 5% BSA for 1 h and then incubated for overnight with anti-MCPIP1 antibody (604421) (R&D), anti-BATF (D7C5) (Cell Signaling Technology), anti-PTPN2 (E-11) (Santa Cruz Biotechnology), anti-SOCS1 (E-9) (Santa Cruz Biotechnology), anti-Hsp90 (MAB3286) (R&D) and anti-β-actin (8H10D10) (Cell Signaling Technology). Membranes were washed 6 times with TBST and then incubated with 1:5,000 diluted HRP conjugated anti-mouse IgG (W4021) (from Promega) for 1 h. Following another 6 times of washes with TBST, the membranes were imaged using the ODYSSEY Fc Analyzer (LI-COR).
Wei J., Long L., Zheng W., Dhungana Y., Lim S.A., Guy C., Wang Y., Wang Y.D., Qian C., Xu B., Anil K., Saravia J., Huang H., Yu J., Doench J.G., Geiger T.L, & Chi H. (2019). Targeting Regnase-1 programs long-lived effector T cells for cancer therapy. Nature, 576(7787), 471-476.
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3

Western Blot Profiling of Immune Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer (ThermoFisher 89900), resolved in 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad 3450124) and transferred to PVDF membrane (Bio-Rad 1620177). Membranes were blocked using 5% BSA for 1 h and then incubated for overnight with anti-MCPIP1 antibody (604421) (R&D), anti-BATF (D7C5) (Cell Signaling Technology), anti-PTPN2 (E-11) (Santa Cruz Biotechnology), anti-SOCS1 (E-9) (Santa Cruz Biotechnology), anti-Hsp90 (MAB3286) (R&D) and anti-β-actin (8H10D10) (Cell Signaling Technology). Membranes were washed 6 times with TBST and then incubated with 1:5,000 diluted HRP conjugated anti-mouse IgG (W4021) (from Promega) for 1 h. Following another 6 times of washes with TBST, the membranes were imaged using the ODYSSEY Fc Analyzer (LI-COR).
Wei J., Long L., Zheng W., Dhungana Y., Lim S.A., Guy C., Wang Y., Wang Y.D., Qian C., Xu B., Anil K., Saravia J., Huang H., Yu J., Doench J.G., Geiger T.L, & Chi H. (2019). Targeting Regnase-1 programs long-lived effector T cells for cancer therapy. Nature, 576(7787), 471-476.
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