The cells were fixed with 4% paraformaldehyde for 15 min, washed three times, washed three times with PBS and blocked with 10% fetal bovine serum for 30 min. The cells were incubated with primary antibodies at 4°C overnight, after which they were incubated with red goat anti-rabbit antibody (Proteintech, Chicago, IL) for 1 h at RT. The following primary antibodies were used: DDR2, 1:200 (ABclonal); COL10A1, 1:200 (BOSTER); E-cadherin, N-cadherin, Vimentin, 1:200 (Cell Signaling Technology). Subsequently, the cells were restained with 4-amino-6-diamino-2-phenylindole (DAPI, Sigma), and images were captured using an Olympus FV1000 confocal laser scanning microscope (Olympus USA, NY, USA) and a Karl Zeiss inverted laser confocal microscope (LSM 880 with Airyscan, Germany).
4 amino 6 diamino 2 phenylindole dapi
Manufactured by Merck Group
Sourced in United States
4-amino-6-diamino-2-phenylindole (DAPI) is a fluorescent stain that binds strongly to DNA. It is commonly used in various laboratory applications for the detection and visualization of nucleic acids.
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Lab products found in correlation
2 protocols using 4 amino 6 diamino 2 phenylindole dapi
1
Immunofluorescence Analysis of Cell Markers
2
Osteoclastogenesis Induction by Scaffold Extracts
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BMMs were seeded in 48-well culture plates at a density of 2 × 104 cells/well and treated with different scaffold extracts in the presence of M-CSF and RANKL for 5 days. The cells were stained with rhodamine-conjugated phalloidin (Abcam, UK) and subsequently stained with 4-amino-6-diamino-2-phenylindole (DAPI; Sigma-Aldrich, USA). Finally, the actin rings were observed and imaged using a fluorescence microscope (Leica, Germany).
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