Dmem f12

Manufactured by Avantor

Sourced in United States

DMEM F12 is a cell culture medium used for the in vitro cultivation of various types of cells. It provides the necessary nutrients and growth factors to support the growth and maintenance of cells in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

N2 supplement, by Avantor (2 mentions) N acetyl l cysteine, by Avantor (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Avantor (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions) L glutamine, by Avantor (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Fb essence, by Avantor (1 mentions) Skov3, by American Type Culture Collection (1 mentions) Ovcar3, by American Type Culture Collection (1 mentions) Sh sy5y, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Roswell park memorial institute (rpmi), by Merck Group (1 mentions) Ovcar4, by American Type Culture Collection (1 mentions) Sh sy5y, by Merck Group (1 mentions) Gentamicin, by Lifeline Cell Technology (1 mentions) Vk2 e6e7 cell line, by American Type Culture Collection (1 mentions)

6 protocols using dmem f12

Isolation and Culture of Rat OL Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The rat OL progenitor cells (OPCs) were propagated as previously described [14 (link)]. In brief, cell suspensions were collected from the cerebral cortex of postnatal 1 to 3 days old SD rats and then cultured in OPCs proliferation medium, containing: DMEM/F12 (Hyclone); 15% B104 conditioned medium; 1% N2 supplements (Invitrogen, USA). After 5 to 7 days, OPCs were isolated via OPCs-isolation medium (DMEM/F12; 0.01% EDTA; 5 mg/mL insulin, 15 to 30 minutes at room temperature and centrifuged at 1000 rpm for 5 minutes. Then, isolated OPCs were cultured with OPCs-proliferation medium in dishes or cover slips coated with poly-D-lysine. For OPCs differentiation experiments, OPCs were incubated with OPCs differentiation medium containing: DMEM/F12; 1% N2 supplement; 5 mg/mL N-acetyl-L-cysteine (Amresco, Solon, OH, USA); 1% fetal bovine serum (FBS); 5 mg/mL insulin. At the distinct time point of differentiation stage, cells were collected and fixed for experiments.

Cao T., Ma T., Xu Y., Tian Y., Cai Q., Li B, & Li H. (2019). Caffeine Treatment Promotes Differentiation and Maturation of Hypoxic Oligodendrocytes via Counterbalancing Adenosine 1 Adenosine Receptor-Induced Calcium Overload. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 1729-1739.

+ Open protocol

+ Expand

Purification and Differentiation of Cortical OPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cortical OPCs were purified as previously described (Niu et al., 2012b (link)). Briefly, the mixed glial cells were isolated from cortex of postnatal day 1–3 neonatal Sprague-Dawley (SD) rats and enriched in OPC growth medium followed by two passages to enrich cell numbers. OPC proliferation medium was DMEM/F12 + 1% N2 supplement + PDGFAA. OPCs were induced to differentiate by replacing the medium with OPC differentiation medium: DMEM/F12 + 1% N2 supplement + 5 mg/mL N-acetyl-L-cysteine (Amresco) + 1% fetal bovine serum + 5 mg/mL insulin.
Reagents used were as follows: Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12; Hyclone, SH30023), N2 supplement (Invitrogen, 17502048), fetal bovine serum (FBS; Hyclone, SV30087), insulin (Sigma, I6634), N-acetyl-L-cysteine (NAC; AMRESCO, 0LA0011), PDGFAA (Peprotech, 100-13A). The SD rats related procedures were performed in accordance with the guidelines approved by the Laboratory Animal Welfare and Ethics Committee of the Third Military Medical University (Niu et al., 2016 (link)).

Li T., Wang L., Ma T., Wang S., Niu J., Li H, & Xiao L. (2018). Dynamic Calcium Release From Endoplasmic Reticulum Mediated by Ryanodine Receptor 3 Is Crucial for Oligodendroglial Differentiation. Frontiers in Molecular Neuroscience, 11, 162.

+ Open protocol

+ Expand

Culturing Embryonic Stem Cells and HEK293T

Check if the same lab product or an alternative is used in the 5 most similar protocols

CyT49 embryonic stem cells were maintained in DMEM F12 (without L-glutamine; VWR) + 10% knockout serum replacement (KSR; Thermo Fisher Scientific), 1% non-essential amino acids (NEAA; Thermo Fisher Scientific), 1% GlutaMAX (Thermo Fisher Scientific), 0.2% β-mercaptoethanol (Thermo Fisher Scientific) and 1% penicillin–streptomycin (Thermo Fisher Scientific). HEK293T were maintained in DMEM F12 containing 100 units/mL penicillin and 100 mg/mL streptomycin sulfate supplemented with 10% foetal bovine serum (FBS).

Vinckier N.K., Patel N.A., Geusz R.J., Wang A., Wang J., Matta I., Harrington A.R., Wortham M., Wetton N., Wang J., Jhala U.S., Rosenfeld M.G., Benner C.W., Shih H.P, & Sander M. (2020). LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development. Nature Communications, 11, 2082.

+ Open protocol

+ Expand

Culturing U87MG and GBM4 Glioblastoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87MG cells were acquired from ATCC‐ and U87MG‐derived cell lines were maintained in DMEM/F12 with 10% FB Essence (VWR, Radnor, PA, USA). GBM4 was obtained from H. Wakimoto (Wakimoto et al., 2009).

Mikheev A.M., Mikheeva S.A., Severs L.J., Funk C.C., Huang L., McFaline‐Figueroa J.L., Schwensen J., Trapnell C., Price N.D., Wong S, & Rostomily R.C. (2018). Targeting TWIST1 through loss of function inhibits tumorigenicity of human glioblastoma. Molecular Oncology, 12(7), 1188-1202.

+ Open protocol

+ Expand

Maintaining and Authenticating Cell Line Panel

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

OVCAR3, OVCAR4, 293T, FT194, FT282, SKOV3, and SH-SY5Y cells were purchased from the American Type Cell Culture (ATCC). HCC5044 and HCC5012 cells were obtained from Dr. Adi Gazdar (Thu et al., 2017 (link)). The ovarian cancer cells were maintained in RPMI (Sigma-Aldrich, R8758) supplemented with 10% fetal bovine serum, 1% GlutaMax (Thermo Fisher Scientific, 35050061) and 1% penicillin/streptomycin. 293T cells were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The fallopian tube cells were cultured in DMEM-F12 (VWR, 45000-346) supplemented with 10% fetal bovine serum. SH-SY5Y cells were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 20% fetal bovine serum and 1% penicillin/streptomycin. Fresh cell stocks were replenished every three months. All cell lines were tested for mycoplasma every 6 months.

Challa S., Khulpateea B.R., Nandu T., Camacho C.V., Ryu K.W., Chen H., Peng Y., Lea J.S, & Lee Kraus W. (2021). Ribosome ADP-Ribosylation Inhibits Translation and Maintains Proteostasis in Cancers. Cell, 184(17), 4531-4546.e26.

+ Open protocol

+ Expand

Maintaining Immortalized Vaginal Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immortalized vaginal epithelial cells (VK2/E6E7 cell line) (ATCC, Rockville, MD) were maintained using basal media supplemented with rh insulin (5 μg/mL), rh epidermal growth factor (ng/mL), L-glutamine (6 mM), epinephrine (1 μM), extract P^™ (0.4%), hydrocortisone hemisuccinate (100 ng/mL), triiodothyronine (10 nM), PS transferrin (μg/mL), phenol red (33 mM), and gentamicin (30 μg/mL) (Lifeline Cell Technology, Frederick, MD). Following trypsinization, cells were neutralized with Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 media (DMEM/F-12, 1:1, VWR) with 10% fetal bovine serum (FBS), and 1x penicillin/streptomycin (100 μg/mL each). For mammalian cell assays, VK2/E6E7 cells were centrifuged at 200 RCF for 5 minutes and resuspended in the media previously described, without addition of gentamicin. Upon counting, cells were seeded into 48-well plates at a density of 25,000 cells per well.

Eilers H., Pernthaler J., Glöckner F.O, & Amann R. (2000). Culturability and In Situ Abundance of Pelagic Bacteria from the North Sea. Applied and Environmental Microbiology, 66(7), 3044-3051.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!