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Hfgf basic fgf2

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HFGF basic/FGF2 is a type of lab equipment produced by Merck Group. It functions as a recombinant human fibroblast growth factor 2 (FGF2) protein. FGF2 is a signaling protein that plays a role in various cellular processes, including cell growth, differentiation, and angiogenesis.

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Lab products found in correlation

L wrn, by American Type Culture Collection (2 mentions) Glutamax, by American Type Culture Collection (2 mentions) Advanced dmem f12, by Thermo Fisher Scientific (2 mentions) B27 supplement, by Thermo Fisher Scientific (2 mentions) Advanced dmem f12, by American Type Culture Collection (2 mentions) B27 supplement, by American Type Culture Collection (2 mentions) Human epidermal growth factor (hegf), by American Type Culture Collection (2 mentions) N2 supplement, by Thermo Fisher Scientific (2 mentions) Human epidermal growth factor (hegf), by Merck Group (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Penicillin streptomycin, by Merck Group (2 mentions) Growth factor reduced matrigel, by Corning (2 mentions)

2 protocols using hfgf basic fgf2

1

Patient-derived Pancreatic and Colorectal Organoids

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The human pancreatic cancer organoids were provided by Dr. David Tuveson (Cold Spring Harbor Laboratory). The patient-derived PDAC organoids hM1A KRASG12D and hT2 KRASG12R were cultured at 37°C in 5% CO2. Cells were seeded in growth factor reduced Matrigel (Corning) domes and fed with complete human feeding medium: advanced DMEM/F12 based WRN condition media (L-WRN (ATCC CRL-3276)), 1x B27 supplement, 10 mM HEPES, 0.01 μM GlutaMAX, 10 mM nicotinamide, 1.25 mM N-acetylcysteine, 50 ng/mL hEGF, 100 ng/mL hFGF10, 0.01 μM hGastrin I, 500 nM A83-01, 1 μM PGE2 and additionally 10.5 μM Y27632 (Boj et al., 2015 (link)). Organoids were tested for mycoplasma. The patient-derived colorectal organoids OT227 KRASG13D, OT238 KRASG12D and OT302 KRASG12D were previously fully characterized in terms of genomic alterations by Schütte et al. (2017) (link). CRC organoids were cultured in crypt culture medium (CCM) containing advanced DMEM/F12 (GIBCO) supplemented with 1x GlutaMAX (GIBCO), 10 mM HEPES buffer (GIBCO), Penicillin/Streptomycin (100 U/ml/100 μg/ml), 1 mM N-acetylcysteine (Sigma), 1x N2 Supplement (GIBCO), 1x B27 supplement (GIBCO) and prepared with freshly added hFGF basic/FGF2 (20 ng/ml) (Sigma) and hEGF (50 ng/ml) (Sigma).
Ozkan-Dagliyan I., Diehl J.N., George S.D., Schaefer A., Papke B., Klotz-Noack K., Waters A.M., Goodwin C.M., Gautam P., Pierobon M., Peng S., Gilbert T.S., Lin K.H., Dagliyan O., Wennerberg K., Petricoin EF I.I.I., Tran N.L., Bhagwat S.V., Tiu R.V., Peng S.B., Herring L.E., Graves L.M., Sers C., Wood K.C., Cox A.D, & Der C.J. (2020). Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant Cancers. Cell reports, 31(11), 107764.
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2

Patient-derived Pancreatic and Colorectal Organoids

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The human pancreatic cancer organoids were provided by Dr. David Tuveson (Cold Spring Harbor Laboratory). The patient-derived PDAC organoids hM1A KRASG12D and hT2 KRASG12R were cultured at 37°C in 5% CO2. Cells were seeded in growth factor reduced Matrigel (Corning) domes and fed with complete human feeding medium: advanced DMEM/F12 based WRN condition media (L-WRN (ATCC CRL-3276)), 1x B27 supplement, 10 mM HEPES, 0.01 μM GlutaMAX, 10 mM nicotinamide, 1.25 mM N-acetylcysteine, 50 ng/mL hEGF, 100 ng/mL hFGF10, 0.01 μM hGastrin I, 500 nM A83-01, 1 μM PGE2 and additionally 10.5 μM Y27632 (Boj et al., 2015 (link)). Organoids were tested for mycoplasma. The patient-derived colorectal organoids OT227 KRASG13D, OT238 KRASG12D and OT302 KRASG12D were previously fully characterized in terms of genomic alterations by Schütte et al. (2017) (link). CRC organoids were cultured in crypt culture medium (CCM) containing advanced DMEM/F12 (GIBCO) supplemented with 1x GlutaMAX (GIBCO), 10 mM HEPES buffer (GIBCO), Penicillin/Streptomycin (100 U/ml/100 μg/ml), 1 mM N-acetylcysteine (Sigma), 1x N2 Supplement (GIBCO), 1x B27 supplement (GIBCO) and prepared with freshly added hFGF basic/FGF2 (20 ng/ml) (Sigma) and hEGF (50 ng/ml) (Sigma).
Ozkan-Dagliyan I., Diehl J.N., George S.D., Schaefer A., Papke B., Klotz-Noack K., Waters A.M., Goodwin C.M., Gautam P., Pierobon M., Peng S., Gilbert T.S., Lin K.H., Dagliyan O., Wennerberg K., Petricoin EF I.I.I., Tran N.L., Bhagwat S.V., Tiu R.V., Peng S.B., Herring L.E., Graves L.M., Sers C., Wood K.C., Cox A.D, & Der C.J. (2020). Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant Cancers. Cell reports, 31(11), 107764.
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