NMR spectroscopy
was used to verify and identify the purity of the esterification product. 1H and 13C NMR were performed on a Bruker Avance
III series equipped with a frequency of 500 MHz and tetramethylsilane
(TMS) as a reference standard to investigate the chemical structure
and synthesis of FAME. The chemical composition of FAME was investigated
by GC–MS. GC was performed on an Agilent model 8890 with polar
columns (DB-WAX & HP-5 MS), UI Agilent column (30 m × 250
μm × 0.25 μm), and split/splitless injectors. The
initial temperature of the oven was 50 °C, and it was increased
at the rate of 5 °C/min until 350 °C. The MS component of
the GC–MS experiment was carried out using an Agilent 5977
MSD apparatus with a mass range of 1.6–1050 amu.
was used to verify and identify the purity of the esterification product. 1H and 13C NMR were performed on a Bruker Avance
III series equipped with a frequency of 500 MHz and tetramethylsilane
(TMS) as a reference standard to investigate the chemical structure
and synthesis of FAME. The chemical composition of FAME was investigated
by GC–MS. GC was performed on an Agilent model 8890 with polar
columns (DB-WAX & HP-5 MS), UI Agilent column (30 m × 250
μm × 0.25 μm), and split/splitless injectors. The
initial temperature of the oven was 50 °C, and it was increased
at the rate of 5 °C/min until 350 °C. The MS component of
the GC–MS experiment was carried out using an Agilent 5977
MSD apparatus with a mass range of 1.6–1050 amu.