Cy and alexa fluor conjugated secondary antibodies

Mouse anti-occludin and rabbit anti-ZO-1 were purchased from Invitrogen. Rabbit anti-cortactin antibody was from Abcam. Rabbit anti-iNOS and eNOS were obtained from Santa Cruz Biotechnology, (Santa Cruz, CA, USA). Mouse anti-prion protein (12F10), for detection of the cellular prion protein was obtained from Cayman (Cayman Chemical, Ann Arbor, MI, USA). Alexa Fluor 488-conjugated phalloidin was purchased from Molecular Probes. Cy and Alexa Fluor-conjugated secondary antibodies were acquired from Jackson Immunoresearch and Molecular Probes, respectively, and used for immunocytochemistry. IR-conjugated secondary antibodies were purchased from LI-COR Biosciences and were used for the in-cell western blot experiments. Unless otherwise mentioned, all other materials used were purchased from Sigma-Aldrich Israel Ltd. (Rehovot, Israel).

Mouse anti-VE-cadherin antibody (sc-9989) diluted 1:50 was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Cy and Alexa Fluor-conjugated secondary antibodies were acquired from Jackson Immunoresearch (Philadelphia, PA, USA) and Molecular Probes (Eugene, OR, USA), respectively, and used for immunocytochemistry. HIF2α inhibitor [51 (link)] (Axon 2034) was obtained from Axon Medchem (Hanzeplein, The Netherlands). Z-VAD-FMK was obtained from Adooq-bioscience (187389-52-2, Irvine, CA, USA) and Santa Cruz Biotechnology (sc-3067, Dallas, TX, USA). Marimastat from Santa Cruz Biotechnology (sc-202223, Dallas, TX, USA), MG-132 from Promega (G9951, Madison, WI, USA). Cytotoxgreen was obtained from Essen BioScience (Ann Arbor, MI, USA) and CellROXgreen from Molecular Probes (Eugene, OR, USA). PX-ethyl was purchased from Sigma (St. Louis, MO, USA). According to the Sigma safety data sheet, safety measures of eye shields, face shields, full-face respirator, and gloves should be taken. PX was used according to our previously reported protocol [43 (link),44 (link)] All other reagents applied in this study were used in accordance to the known literature and the supplier’s guidelines.