Htyr (≥98%) was purchased from Extrasynthèse (Genay, France) and Tyr (≥98%) from Alfa Aesar GmdH & Co KG, (Karlsruhe, Germany), CA (98%) and DHPA (98%) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Analytical-grade methanol, HPLC grade methanol, acetonitrile and water were obtained from ChemLab (Zeldegem, Belgium). Phosphoric acid (≥85%) and sulphuric acid (95–98%) were supplied by Sigma-Aldrich (Steinheim, Germany). Sodium carbonate anhydrous and Folin-Ciocalteu (F-C) reagent were obtained from ChemLab (Zeldegem, Belgium). Polyvinylidene fluoride (PVDF) membrane filters (0.22 µm) were from Schleicher & Schuell, (Dassel, Germany).
Hplc grade methanol
Manufactured by Chem-Lab
Sourced in Belgium
HPLC grade methanol is a high-purity solvent used in high-performance liquid chromatography (HPLC) applications. It is a clear, colorless liquid with a specific gravity of approximately 0.791 at 20°C. HPLC grade methanol meets stringent purity requirements for use in analytical and research laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Chem-Lab (2 mentions)
Sodium carbonate anhydrous, by Chem-Lab (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
Sulphuric acid, by Merck Group (1 mentions)
Folin ciocalteu f c reagent, by Chem-Lab (1 mentions)
Phenol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Chem-Lab (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by ITW Reagents (1 mentions)
Formic acid, by ITW Reagents (1 mentions)
Tartaric acid, by ITW Reagents (1 mentions)
Glacial acetic acid, by Avantor (1 mentions)
Iron 3 chloride hexahydrate, by Merck Group (1 mentions)
Trolox s 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid, by J&K Scientific (1 mentions)
Dpph 2 2 diphenyl 1 picrylhydrazyl, by Merck Group (1 mentions)
Gallic acid, by J&K Scientific (1 mentions)
Folin ciocalteu reagent, by Chem-Lab (1 mentions)
Benzaldehyde, by Merck Group (1 mentions)
6 protocols using hplc grade methanol
1
Quantitative Analysis of Bioactive Compounds
2
Analytical Reagent Procurement for Research
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HPLC grade methanol, acetonitrile, and isopropanol were obtained from Chem-Lab (Zedelgem, Belgium), while phenol and GABA standards were obtained from Sigma Aldrich (St. Louis, MO, USA). Analytical grade anhydrous ethanol and sodium hydroxide were purchased from Thermo Fisher Scientific (Waltham, MA, USA). GO standards were obtained from TCI Europe (Zwijndrecht, Belgium).
3
Analytical Procedures for Food Compounds
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All chemicals used had a purity grade higher than 97%. Ethyl acetate was purchased from Fisher Scientific (Leicestershire, UK). Formic acid, tartaric acid, and sodium hydroxide were obtained from Panreac (Barcelona, Spain) and HPLC-grade methanol was from Chem-Lab (Zedelgem, Belgium). Sotolon food grade standard and absolute ethanol were purchased from Sigma-Aldrich (St. Louis, MA, USA). Ultra-pure water (type 1) was obtained from a Simplicity® UV ultra-pure water apparatus from Millipore (Milford, MA, USA).
4
Fatty Acid Analysis Protocol
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FFA (≥98 %) was obtained from Fluka Chemicals (Basel, Switzerland). 2‐FA (98%) and HMF (99%) were obtained from Aldrich Chemistry (St. Louis, MO, USA). HMFA was obtained from Matrix Scientific (Columbia, SC, USA). Methanol (HPLC grade) was obtained from Chem Lab NV (Zedelgem, Belgium). Water for HPLC was obtained from Ultra clear TWF‐UV system (Siemens water technologies, Germany). Glacial acetic acid (99–100%) was obtained from Avantor performance material (Deventer, Netherlands).
5
Wheat Flour and Chlorella Characterization
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Wheat flour for breadmaking (12.07% of protein (N × 5.7), 12.9% moisture) was purchased from Harinera la Meta S.A. (Valencia, Spain) . Commercial Chlorella vulgaris microalga flour was purchased from Allmicroalgae (Lisbon, Portugal). The DPPH (2,2-diphenyl-1-picrylhydrazyl) was from Sigma Aldrich, USA, whereas TPTZ (2,4,6-tripyridyl-s-triazine) was obtained from Alfa Aesar, GmbH & Go KG, Karlsruhe, Germany. ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), Trolox ((S)-(-)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), and gallic acid were from J&K Scientific GmbH, Pforzheim, Germany. Folin-Ciocalteu reagent, and methanol (HPLC grade) were obtained from Chem-Lab NV, Zedelgem, Belgium. Iron (III) chloride hexahydrate was from Merk, KGaA, Darmstadt, Germany. All the other chemicals used were of analytical grade.
6
Chitosan-Based Biomaterial Synthesis
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Chitosan (1.0–3.0 × 105 g mol−1 molecular weight, 98% degree of deacetylation, purity 98%, and ash ≤ 2%) was made from the shells of shrimps and crabs Pandalus borealis and purchased from Acros Organics (Morris Plains, NJ, USA). Methanol (HPLC grade) was supplied by chem-lab, (Zedelgem, Belgium). Ethanol, epichlorohydrin, and hydrochloric acid were obtained from PanReac. AppliChem- ITW Reagent (Darmstadt, Germany ). Sodium carbonate and sodium hydroxide pellets were obtained from Loba Chemie, (Mumbai, India). Six-amino-1,3-dimethyluracil, benzaldehyde, 2-chlorobenzaldehyde, were purchased from Sigma-Aldrich, (Munich, Germany). Congo red dye (Figure 1 ) was supplied by Winlab (Leicestershire, UK). The buffer powder pillows of pH 4, 7 and 9 were supplied by Hach Company (Loveland, CO, USA).
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